Morphological and molecular characterisation of Eimeria vison-like oocysts (Apicomplexa:Eimeriidae) in farmed mink (Neovison vison) in Denmark

A survey was conducted on 30 Danish mink farms from April to October 2016 to determine the prevalence and species of Eimeria in Danish farmed mink. In total, 2.6% of mink faecal samples (108/4140) were positive for Eimeria vison -like oocysts by microscopy, with 24.8% (78/315) of mink being positive...

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Bibliographic Details
Published in:Parasitology research (1987) 2018-09, Vol.117 (9), p.2933-2939
Main Authors: Petersen, H. H., Yang, R., Chriél, M., Hansen, M. S., Ryan, U. M.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Coccidiosis - epidemiology
Coccidiosis - parasitology
Coccidiosis - veterinary
Cytochrome-c oxidase
Denmark - epidemiology
Eimeria
Eimeria - classification
Eimeria - genetics
Eimeria - isolation & purification
Electron Transport Complex IV - genetics
Farms
Feces - parasitology
Genotypes
Immunology
Medical Microbiology
Mice
Microbiology
Mink - parasitology
Morphology
Oocysts
Oocysts - classification
Oocysts - isolation & purification
Oocysts - physiology
Original Paper
Phylogenetics
Phylogeny
Polymerase Chain Reaction - veterinary
RNA, Ribosomal, 18S - genetics
rRNA 18S
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Summary:A survey was conducted on 30 Danish mink farms from April to October 2016 to determine the prevalence and species of Eimeria in Danish farmed mink. In total, 2.6% of mink faecal samples (108/4140) were positive for Eimeria vison -like oocysts by microscopy, with 24.8% (78/315) of mink being positive at least once during the study period. Morphological analysis of sporulated oocysts ( n  = 20) identified Eimeria vison -like oocysts measuring 21.0 × 13.8 μm with a length/width ( L / W ) ratio of 1.5. Phylogenetic analysis of 18S rRNA sequences (1221 bp) from three positive mink indicated that Eimeria vison -like shared the highest genetic similarity to Eimeria sp. ex Apodemus agrarius from a Striped field mouse ( A. agrarius ) from the Czech Republic (99.6%). Analysis of a shorter region of 18S (531 bp) revealed that the E. vison -like genotype sequences grouped in the same clade and shared 97.7% similarity with E. furonis . At the cytochrome c oxidase subunit I (COI) locus, mink-derived sequences were not available from GenBank and phylogenetic analysis placed the novel E. vison -like in a clade with E. cf. ictidea (99.4% similarity) from a black footed ferret ( Mustela nigripes ) from Canada.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-018-5989-1