Enzymatic film formation of nature-derived phenolic amines

An enzyme-instructed method is developed for material-independent, cytocompatible coating of phenolic amines, inspired by melanogenesis found in nature. Tyrosinase-based film formation proceeds smoothly in an aqueous solution at neutral pH, and can use various phenolic amines including catecholamine...

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Bibliographic Details
Published in:Nanoscale 2018-01, Vol.10 (28), p.13351-13355
Main Authors: Kim, Ji Yup, Kim, Won Il, Youn, Wongu, Seo, Jeongyeon, Kim, Beom Jin, Lee, Jungkyu K, Choi, Insung S
Format: Article
Language:English
Subjects:
Amines
Biocompatibility
Biomedical materials
Dopamine
Norepinephrine
Organic chemistry
Tyrosinase
Tyrosine
Viability
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Summary:An enzyme-instructed method is developed for material-independent, cytocompatible coating of phenolic amines, inspired by melanogenesis found in nature. Tyrosinase-based film formation proceeds smoothly in an aqueous solution at neutral pH, and can use various phenolic amines including catecholamines, such as tyrosine, tyramine, dopamine, norepinephrine, and DOPA, as a coating precursor. Compared with polydopamine coating, the method is fast and efficient, and forms uniform films. Its high cytocompatibility is advantageously applied to cell-surface engineering, where chemically labile Jurkat cells are coated individually without any noticeable decrease in viability. Considering the huge potential of polyphenolic-based coatings, the strategy developed herein will provide an advanced tool for manipulating biological entities, including living cells, in biomedical and medicinal applications.
ISSN:2040-3364
2040-3372
DOI:10.1039/c8nr04312d