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Novel investigation of the performance of continuous packed bed bioreactor (CPBBR) by isolated Bacillus sp. M4 and proteomic study
[Display omitted] •Isolation and characterization of benzene degradating Bacillus sp. M4.•Performance of continuous packed bed bioreactor was evaluated.•Proteomic study for benzene biodegradation was performed.•Docking calculation confirmed the benzene biodegradation. The present study reveals the b...
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Published in: | Bioresource technology 2018-10, Vol.266, p.335-342 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | [Display omitted]
•Isolation and characterization of benzene degradating Bacillus sp. M4.•Performance of continuous packed bed bioreactor was evaluated.•Proteomic study for benzene biodegradation was performed.•Docking calculation confirmed the benzene biodegradation.
The present study reveals the benzene degrading potential of bacterial species isolated from petroleum contaminated soil. Genomic analysis suggests that Bacillus sp. M4 was found to be dominating species. The process parameters were optimized and found to be pH 7.0 ± 0.2, temperature 32 ± 5 °C, immobilization time (20 days) and benzene concentration 400 mg/L. The maximum removal efficiency of benzene was calculated and found to be 93.13% at elimination capacity156 (mg/L/day) and inlet loading rate 192 (mg/L/day) achieved in 54th days of operation. In the study, the residual metabolites were analyzed by GC/MS analysis and identified as benzene-1,2-diol. In order to identify the responsible protein involved in the process of benzene biodegradation The proteomic study was performed and proteins were identified by MALDI-TOF analysis. The molecular docking was confirmed by the benzene biodegradation. |
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ISSN: | 0960-8524 1873-2976 |
DOI: | 10.1016/j.biortech.2018.06.064 |