A many probes-one spot hybridization oligonucleotide microarray

A many probes-one spot hybridization oligonucleotide microarray

A variant of the hybridization oligonucleotide microarray, utilizing the principle of many probes-one spot (MPOS-microarrays), is proposed. A case study based on Orthopoxviruses ( Variola , Monkeypox , and Ectromelia viruses ) demonstrates a considerable increase in the fluorescence signal (up to 10...

Full description

Saved in:
Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2018-09, Vol.410 (23), p.5817-5823
Main Authors: Kostina, Elena V., Sinyakov, Alexander N., Ryabinin, Vladimir A.
Format: Article
Language:eng
Subjects:
Analysis
Analytical Chemistry
Biochemistry
Biology
Biosensors
Case studies
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
CRISPR
Deoxyribonucleic acid
DNA
DNA microarrays
DNA probes
DNA viruses
Drug resistance
Ecological monitoring
Fluorescence
Food Science
Gene expression
Genetic testing
Hybridization
Identification and classification
Influenza
Laboratory Medicine
Microorganisms
Monitoring/Environmental Analysis
Monkeypox
Nucleic acid hybridization
Oligonucleotides
Orthopoxvirus
Probes
Research Paper
Smallpox
Viruses
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A variant of the hybridization oligonucleotide microarray, utilizing the principle of many probes-one spot (MPOS-microarrays), is proposed. A case study based on Orthopoxviruses ( Variola , Monkeypox , and Ectromelia viruses ) demonstrates a considerable increase in the fluorescence signal (up to 100-fold) when several oligonucleotide probes are printed to one spot. Moreover, the specificity of detection also increases (almost 1000-fold), allowing the use of probes that individually lack such high specificity. The optimal probes have a Tm of 32–37 °C and length of 13–15 bases. We suggest that the high specificity and sensitivity of the MPOS-microarray is a result of cooperativity of DNA binding with all probes immobilized in the spot. This variant of DNA detection can be useful for designing biosensors, tools for point-of-care (POC) diagnostics, microbial ecology, analysis of clustered regularly interspaced short palindromic repeats (CRISPR), and others. Graphical abstract ᅟ
ISSN:1618-2642
1618-2650