Binding of Mucin by E. coli from Human Gut

Cells of E. coli isolates from the gut of healthy volunteers ( N =5) and patients with Crohn’s disease ( N =5) and laboratory E. coli strain DH5α bound mucin in vitro in similar amounts ranging from 0.02 to 0.12 mg/mg of bacterial dry weight. Binding was evaluated by the decrease in optical absorpti...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2018-06, Vol.165 (2), p.235-238
Main Authors: Vakhrusheva, T. V., Baikova, Yu. P., Balabushevich, N. G., Gusev, S. A., Lomakina, G. Yu, Sholina, E. A., Moshkovskaya, M. A., Shcherbakov, P. L., Pobeguts, O. V., Mikhal’chik, E. V.
Format: Article
Language:English
Subjects:
Analysis
Bacteria
Bacterial Adhesion
Biomedical and Life Sciences
Biomedicine
Cell Biology
Confocal microscopy
Crohn Disease - metabolism
Crohn Disease - microbiology
Crohn Disease - pathology
E coli
Escherichia coli - isolation & purification
Escherichia coli - metabolism
Feces - microbiology
Gastrointestinal Microbiome
Healthy Volunteers
Humans
Internal Medicine
Intestines - microbiology
Intestines - pathology
Laboratory Medicine
Mucin
Mucins - metabolism
Pathology
Protein Binding
Sodium chloride
Zeta potential
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Summary:Cells of E. coli isolates from the gut of healthy volunteers ( N =5) and patients with Crohn’s disease ( N =5) and laboratory E. coli strain DH5α bound mucin in vitro in similar amounts ranging from 0.02 to 0.12 mg/mg of bacterial dry weight. Binding was evaluated by the decrease in optical absorption of mucin solution at 214 nm after incubation with bacteria. Detailed analysis of mucin binding by one of isolates showed that during incubation of 0.09 mg/ml bacteria in 0.15 M NaCl containing 0.1 mg/ml mucin at 25 o C, maximum binding was reached in 30 min, while in the presence of 14 mM α-methyl mannoside, mucin binding decreased by 46% ( p
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-018-4137-3