Genotyping of thermotolerant Campylobacter from poultry slaughterhouse by amplified fragment length polymorphism

To examine the occurrence, diversity and transmission of Campylobacter in a poultry slaughterhouse. During a 4-week period, a slaughterhouse was sampled alternately during slaughtering and the following mornings post-disinfection. Samples were taken from poultry at six stages in the slaughter proces...

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Bibliographic Details
Published in:Journal of applied microbiology 2007-08, Vol.103 (2), p.271-279
Main Authors: Johnsen, G, Kruse, H, Hofshagen, M
Format: Article
Language:English
Subjects:
Abattoirs
amplified fragment length polymorphism
Amplified Fragment Length Polymorphism Analysis - methods
Animals
Biological and medical sciences
Campylobacter
Campylobacter - genetics
Campylobacter - isolation & purification
Campylobacter coli - genetics
Campylobacter coli - isolation & purification
Campylobacter Infections - microbiology
Campylobacter Infections - veterinary
Campylobacter jejuni - genetics
Campylobacter jejuni - isolation & purification
Campylobacter lari - genetics
Campylobacter lari - isolation & purification
Cecum - microbiology
Chickens - genetics
Environmental Pollution
Female
Food Microbiology
Fundamental and applied biological sciences. Psychology
Genotype
genotyping
Hot Temperature
Microbiology
Polymorphism, Genetic - genetics
poultry
Poultry Diseases - microbiology
slaughterhouse
slaughterhouses
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Summary:To examine the occurrence, diversity and transmission of Campylobacter in a poultry slaughterhouse. During a 4-week period, a slaughterhouse was sampled alternately during slaughtering and the following mornings post-disinfection. Samples were taken from poultry at six stages in the slaughter process and from 25 environmental sites. For positive broiler flocks slaughtered on one occasion, 92% and 48% of the environmental sites were positive during slaughter and post-disinfection, respectively. For positive laying hen flocks slaughtered on three occasions, 8-56% and 12-20% of the environmental sites were positive during slaughter and post-disinfection, respectively. Genetic fingerprinting by amplified fragment length polymorphism (AFLP) of the 109 isolates obtained resulted in 28 different AFLP clones. Five AFLP clones were present for more than 1 week. Slaughtering of Campylobacter-positive broilers resulted in extensive contamination of the slaughterhouse, including the air. A high proportion of the laying hen flocks were Campylobacter positive, but these caused less environmental contamination than the broilers. This, together with the freezing of all layer carcasses, results in a lower public health risk from laying hens, when compared with broilers. When slaughtering Campylobacter-positive broilers, the implementation of preventive measures is important to reduce contamination of negative carcasses and to protect the workers against infection.
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2006.03238.x