Influence of aqueous crude extracts of medicinal plants on the osmotic stability of human erythrocytes

This work analyzed the effects of the aqueous crude extracts of Artemisia absinthium L., Lippia sp., Bryophyllum sp., Solidago microglossa DC, Cymbopogon citratus DC and Mentha x villosa HUDSON on the osmotic stability of human erythrocytes. Hemolysis was monitored by measurement of absorbance at 54...

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Bibliographic Details
Published in:Toxicology in vitro 2008-02, Vol.22 (1), p.219-224
Main Authors: de Freitas, Mariana V., Netto, Rita de Cássia M., da Costa Huss, Juliana C., de Souza, Tatiana Maria T., Costa, Júnia O., Firmino, Cynthia B., Penha-Silva, Nilson
Format: Article
Language:English
Subjects:
Adolescent
Adult
Artemisia absinthium
Artemisia absinthium - chemistry
Crassulaceae - chemistry
Cymbopogon - chemistry
Cymbopogon citratus
Erythrocyte Membrane - drug effects
Erythrocyte Membrane - metabolism
Erythrocytes
Hematotoxicity
Hemolysis - drug effects
Humans
In Vitro Techniques
Lippia
Lippia - chemistry
Medicinal plants
Membrane stability
Mentha
Mentha - chemistry
Osmotic Fragility - drug effects
Plant Extracts - toxicity
Plants, Medicinal - chemistry
Regression Analysis
Solidago
Solidago - chemistry
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Summary:This work analyzed the effects of the aqueous crude extracts of Artemisia absinthium L., Lippia sp., Bryophyllum sp., Solidago microglossa DC, Cymbopogon citratus DC and Mentha x villosa HUDSON on the osmotic stability of human erythrocytes. Hemolysis was monitored by measurement of absorbance at 540 nm following addition of erythrocytes to NaCl solutions of varying concentration. Absorbance was fitted to sigmoid regression curves given by the Boltzmann equation, and hemolysis was characterized by the NaCl concentration leading to lysis of 50% of cells ( H 50), and by the intensity ( H) and the amplitude (dS) of the lysis effect. The parameters were determined in the absence and presence of the crude extracts. The extracts of Artemisia absinthium, Lippia sp., C. citratus and M. villosa protected human erythrocytes against hypotonic shock, as evidenced by a decrease in the values of H and H 50 compared to the control solution ( p < 0.05). The extracts of Bryophyllum sp. and S. microglossa enhanced hemolysis, since their H 50 values were higher than in the control group ( p < 0.05), but they also showed protective effects, as evidenced by a decrease in H and an increase in dS.
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2007.07.010