Supercritical fluid extraction of cordycepin and adenosine from Cordyceps kyushuensis and purification by high-speed counter-current chromatography

Supercritical fluid extraction (SFE) was used to extract cordycepin and adenosine from Cordyceps kyushuensis. An orthogonal array design (OAD) test, L 9(3) 4, including pressure, temperature, the mount of modifier and the flow rate of CO 2 was performed to get the optimal conditions. The process was...

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Bibliographic Details
Published in:Separation and purification technology 2009-05, Vol.66 (3), p.625-629
Main Authors: Ling, Jian Ya, Zhang, Guo Ying, Lin, Jian Qun, Cui, Zhao Jie, Zhang, Chang Kai
Format: Article
Language:English
Subjects:
Adenosine
Applied sciences
Chemical engineering
Cordycepin
Cordyceps kyushuensis
Exact sciences and technology
High-speed counter-current chromatography
Liquid-liquid extraction
Supercritical fluid extraction
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Summary:Supercritical fluid extraction (SFE) was used to extract cordycepin and adenosine from Cordyceps kyushuensis. An orthogonal array design (OAD) test, L 9(3) 4, including pressure, temperature, the mount of modifier and the flow rate of CO 2 was performed to get the optimal conditions. The process was then scaled up by 30 times with a preparative SFE system under 40 MPa, 40 °C, the mount of modifier (0.04 ml min −1) and a flow rate of CO 2 (2.0 l min −1). The preparative SFE extracts were separated and purified by high-speed counter-current chromatography (HSCCC) with a selected two-phase solvent system composed of ethyl acetate–n-butyl alcohol–water at an optimized volume ratio of 1:4:5, and the collected fractions were analyzed by high-performance liquid chromatography (HPLC). Two nucleosides were obtained and yielded 8.92 mg of cordycepin, 5.94 mg of adenosine with purities of 98.5 and 99.2% from 400 mg SFE crude extraction, in one-step separation, respectively.
ISSN:1383-5866
1873-3794
DOI:10.1016/j.seppur.2008.12.022