Mapping and Confirmation of the 'Hyuuga' Red-Brown Lesion Resistance Gene for Asian Soybean Rust

Asian soybean rust (ASR), caused by Phakopsora pachyrhizi Syd., is a widespread disease of soybean [Glycine max (L.) Merr.] with the potential to cause serious economic losses. The objective of this study was to genetically map red-brown lesion type resistance from the cultivar Hyuuga. A population...

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Bibliographic Details
Published in:Crop science 2007-03, Vol.47 (2), p.829-834
Main Authors: Monteros, M.J, Missaoui, A.M, Phillips, D.V, Walker, D.R, Boerma, H.R
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
chromosome mapping
Crop diseases
Cultivars
disease resistance
field experimentation
Fundamental and applied biological sciences. Psychology
genetic markers
genetic resistance
Genetics
Genetics and breeding of economic plants
Genomics
germplasm
germplasm screening
Glycine max
inbred lines
lesions (plant)
marker-assisted selection
microsatellite repeats
molecular sequence data
Phakopsora pachyrhizi
plant genetic resources
rust diseases
Soybeans
sporulation
Varietal selection. Specialized plant breeding, plant breeding aims
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Summary:Asian soybean rust (ASR), caused by Phakopsora pachyrhizi Syd., is a widespread disease of soybean [Glycine max (L.) Merr.] with the potential to cause serious economic losses. The objective of this study was to genetically map red-brown lesion type resistance from the cultivar Hyuuga. A population of 117 recombinant inbred lines (RILs) from the cross of Dillon (tan lesion) x Hyuuga (red-brown [RB] lesion) was rated for ASR lesion type in the field and inoculated with P. pachyrhizi in the greenhouse. The RB resistance gene mapped between Satt460 and Satt307 on linkage group (LG)-C2. When field severity and lesion density in the greenhouse were mapped, the Rpp?(Hyuuga) locus explained 22 and 15% of the variation, respectively (P < 0.0001). The RB lesion type was associated with lower severity, fewer lesions, and reduced sporulation when compared to the tan lesion type. A population from the cross of Benning x Hyuuga was screened with simple sequence repeat (SSR) markers in the region on LG-C2 flanked by Satt134 and Satt460. Genotype at these markers was used to predict lesion type when the plants were exposed to P. pachyrhizi. All the lines selected for the Hyuuga markers in this interval had the RB lesion type and they averaged approximately 50% fewer lesions compared to lines with tan lesions. Sporulation was only detected in 6% of the RB lines compared with 100% of the tan lines. Marker-assisted selection can be used to develop soybean cultivars with the Rpp?(Hyuuga) gene for resistance to ASR.
ISSN:0011-183X
1435-0653
DOI:10.2135/cropsci06.07.0462