Lime pretreatment, enzymatic saccharification and fermentation of rice hulls to ethanol

Rice hulls used in this study contained 35.6±0.1% cellulose and 12.0±0.7% hemicellulose. The maximum yield of monomeric sugars from rice hulls (15.0%, w/v) by lime pretreatment (100 mg g −1 hulls, 121 °C, 1 h) and enzymatic saccharification (45 °C, pH 5.0, 72 h) using a cocktail of three commercial...

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Bibliographic Details
Published in:Biomass & bioenergy 2008-10, Vol.32 (10), p.971-977
Main Authors: Saha, Badal C., Cotta, Michael A.
Format: Article
Language:English
Subjects:
Alcohols: methanol, ethanol, etc
Alternative fuels. Production and utilization
Applied sciences
Energy
Enzymatic saccharification
Escherichia coli
Ethanol
Exact sciences and technology
Fuels
Lime pretreatment
Oryza sativa
Rice hulls
Separate hydrolysis and fermentation
Simultaneous saccharification and fermentation
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Summary:Rice hulls used in this study contained 35.6±0.1% cellulose and 12.0±0.7% hemicellulose. The maximum yield of monomeric sugars from rice hulls (15.0%, w/v) by lime pretreatment (100 mg g −1 hulls, 121 °C, 1 h) and enzymatic saccharification (45 °C, pH 5.0, 72 h) using a cocktail of three commercial enzyme preparations (cellulase, β-glucosidase and hemicellulase) at the dose level of 0.15 ml of each enzyme preparation g −1 hulls was 154±1 mg g −1 (32% yield). The lime pretreatment did not generate any detectable furfural and hydroxymethyl furfural in the hydrolyzate. The concentration of ethanol from lime-pretreated enzyme-saccharified rice hull (138 g) hydrolyzate by recombinant Escherichia coli strain FBR5 at pH 6.5 and 35 °C in 19 h was 9.8±0.5 g l −1 with a yield of 0.49 g g −1 available sugars. The ethanol concentration was 11.0±1.0 g l −1 in the case of simultaneous saccharification and fermentation by the E. coli strain at pH 6.0 and 35 °C in 53 h.
ISSN:0961-9534
1873-2909
DOI:10.1016/j.biombioe.2008.01.014