Investigation of the Persistence of Penicillin G and Dihydrostreptomycin Residues in Milk of Lactating Buffaloes (Bubalus bubalis) Using Ultra-High-Performance Liquid Chromatography and Tandem Mass Spectrometry

The purpose of this research was to evaluate the persistence of penicillin G and dihydrostreptomycin in milk of lactating buffaloes following intramuscular injection of procaine penicillin G (200000 IU/mL) and dihydrostreptomycin sulfate (250 mg/mL) every 24 h for 3 days. Milk samples were collected...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2018-06, Vol.66 (25), p.6388-6393
Main Authors: Chirollo, Claudia, Vassallo, Antonio, Dal Piaz, Fabrizio, Lamagna, Barbara, Tortora, Gennaro, Neglia, Gianluca, De Tommasi, Nunziatina, Severino, Lorella
Format: Article
Language:English
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Summary:The purpose of this research was to evaluate the persistence of penicillin G and dihydrostreptomycin in milk of lactating buffaloes following intramuscular injection of procaine penicillin G (200000 IU/mL) and dihydrostreptomycin sulfate (250 mg/mL) every 24 h for 3 days. Milk samples were collected twice daily up to the 13th milking post-treatment and analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry. The analytical method has been validated according to Commission Decision 2002/657/EC. The highest concentrations of penicillin G (275 μg kg–1) and dihydrostreptomycin (220.5 μg kg–1) were detected in the milk of the first milkings post-treatment, and levels were below the maximum residue limit of 4 and 200 μg kg–1 in all treated buffaloes at milkings 12 and 2, respectively. The results of this study demonstrate that a nine-milking withdrawal time set for bovine milk was not adequate for depletion of penicillin G in lactating buffaloes.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.8b00229