Preparation and protective efficacy of a chicken embryo kidney cell-attenuation GI-19/QX-like avian infectious bronchitis virus vaccine

•A CEK-attenuated QX-like IBV vaccine SczyC100 was prepared.•SczyC100 is effective against prevalent Chinese IBV strains.•The vaccine efficacy of SczyC100 is significantly better than H120. Avian infectious bronchitis (IB) is a highly contagious disease, and hazardous to the poultry industry. Immune...

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Bibliographic Details
Published in:Vaccine 2018-06, Vol.36 (28), p.4087-4094
Main Authors: Xia, Jing, He, Xiao, Du, Li-Jing, Liu, Yue-Yue, You, Guo-Jin, Li, Shu-Yun, Liu, Ping, Cao, San-Jie, Han, Xin-Feng, Huang, Yong
Format: Article
Language:English
Subjects:
Animals
Attenuation
Avian infectious bronchitis
Bronchitis
CEK adaptation
Cell Line
Chickens
China
Coronavirus Infections - immunology
Coronavirus Infections - pathology
Coronavirus Infections - prevention & control
Coronavirus Infections - veterinary
Design
Disease
Eggs
Embryos
Epithelial Cells - virology
Infections
Infectious bronchitis disease
Infectious bronchitis virus - growth & development
Infectious bronchitis virus - immunology
Infectious bronchitis virus - pathogenicity
Infectious diseases
Influenza
Kidneys
Laboratory animals
Morbidity
Mortality
Pathogenicity
Pathogens
Poultry
Poultry Diseases - immunology
Poultry Diseases - prevention & control
Protective
Reversion
RNA polymerase
Safety
Serial Passage
Serotypes
Strains (organisms)
Survival Analysis
Thermal cycling
Trachea
Trachea - virology
Vaccine
Vaccines
Vaccines, Attenuated - administration & dosage
Vaccines, Attenuated - immunology
Vaccines, Attenuated - isolation & purification
Veterinary medicine
Viral Load
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
Viral Vaccines - isolation & purification
Virulence
Virus Cultivation - methods
Viruses
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Summary:•A CEK-attenuated QX-like IBV vaccine SczyC100 was prepared.•SczyC100 is effective against prevalent Chinese IBV strains.•The vaccine efficacy of SczyC100 is significantly better than H120. Avian infectious bronchitis (IB) is a highly contagious disease, and hazardous to the poultry industry. Immune failure often occurs due to the emergence of new serotypes or field strains antigenically different from the vaccine strains. To prepare a candidate vaccine against the prevalent avian infectious bronchitis virus (IBV) in China, the GI-19/QX-like field isolate Sczy3 was selected as the progenitor strain and attenuated via passaging in chicken embryo kidney (CEK) cells for 100 times. The 100th generation of CEK-adapted strain, designated SczyC100, was safe to use on one-day old specific pathogen-free (SPF) chicken as determined by pathogenicity and virulence reversion test. The efficacies of SczyC100 and two commonly used commercial vaccines (H120 and 4/91) against prevalent GI-19/QX and GI-7/TWI type virulent strains were evaluated. Sczy3C100 effectively reduced the morbidity, mortality, mean lesion scores (MLSs), and viral load of trachea of chickens challenged by GI-19/QX and GI-7/TWI strains. CEK-adapted SczyC100 is therefore a potential vaccine candidate for the control of IB in China.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2018.05.094