Determination of PCDD/Fs and dioxin-like PCBs in fish oils for feed ingredients by congener-specific chemical analysis and CALUX bioassay

The present research was intended to determine the suitability of the CALUX assay as a screening method for dioxins in fish oil used as a feed ingredient in Japan. Alteration of TEQ in fish oil according to newly proposed toxic equivalency factors (TEF) is also discussed. In the analysis, polychlori...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2007-10, Vol.69 (8), p.1188-1194
Main Authors: Hasegawa, Jun, Guruge, Keerthi S., Seike, Nobuyasu, Shirai, Yuji, Yamata, Toshiaki, Nakamura, Masafumi, Handa, Hiroshi, Yamanaka, Noriko, Miyazaki, Shigeru
Format: Article
Language:English
Subjects:
Animal Feed
bioassays
Biological and medical sciences
Biological Assay - methods
CALUX assay
cell lines
chemical analysis
chemical residues
congeners
cytotoxicity
Dioxin-like PCBs
dioxins
Environmental Monitoring - methods
feed contamination
feeds
Fish oil
fish oils
Fish Oils - chemistry
Food toxicology
Gas Chromatography-Mass Spectrometry
hepatoma
Japan
Luciferases - analysis
Medical sciences
mice
PCDD/Fs
polychlorinated biphenyls
Polychlorinated Biphenyls - analysis
polychlorinated dibenzodioxins
Polychlorinated Dibenzodioxins - analogs & derivatives
Polychlorinated Dibenzodioxins - analysis
polychlorinated dibenzofurans
Toxicology
WHO-TEQ
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Summary:The present research was intended to determine the suitability of the CALUX assay as a screening method for dioxins in fish oil used as a feed ingredient in Japan. Alteration of TEQ in fish oil according to newly proposed toxic equivalency factors (TEF) is also discussed. In the analysis, polychlorinated dibenzo- p-dioxins and dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) in 41 fish oil samples were determined by using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS) and CALUX bioassay. The mean TEQ values derived from 1998 WHO-TEF of PCDD/Fs and DL-PCBs were 2.6 and 9.9 pg g −1 (ww), respectively. The levels of TEQ derived from the recently re-evaluated 2005 WHO-TEF were slightly lower than those of the former in both groups. Notably, the contribution of mono-ortho DL-PCBs to total 2005 WHO-TEQ was considerably decreased compared to the case of 1998 WHO-TEQ, resulting from the reduction in its TEF values, while the non-ortho DL-PCBs contribution was increased. The mean TEQ determined by CALUX assay for PCDD/Fs was approximately three times higher, whereas DL-PCBs was approximately two times lower than WHO-TEQ determined by HRGC/HRMS; the sum of PCDD/Fs and DL-PCBs was very similar by both methods. The correlation coefficients of TEQ between the CALUX assay and HRGC/HRMS analysis were 0.84, 0.89, and 0.90 for PCDD/Fs, DL-PCBs, and the sum, respectively. These results suggest that the CALUX assay is a very useful method for the screening of dioxin-related compounds in fish oils.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2007.06.021