A conditional transposon-based insertional mutagenesis screen for genes associated with mouse hepatocellular carcinoma

We describe a system that permits conditional mobilization of a Sleeping Beauty (SB) transposase allele by Cre recombinase to induce cancer specifically in a tissue of interest. To demonstrate its potential for developing tissue-specific models of cancer in mice, we limit SB transposition to the liv...

Full description

Bibliographic Details

Published in:	Nature biotechnology 2009-03, Vol.27 (3), p.264-274
Main Authors:	Akagi, Keiko, Sarver, Aaron, Keng, Vincent W, Chiang, Derek Y, Jenkins, Nancy A, Tessarollo, Lino, Dupuy, Adam J, Lowe, Scott W, Largaespada, David A, Matise, Ilze, Silverstein, Kevin A T, Starr, Timothy K, Collier, Lara S, Powers, Scott, Copeland, Neal G, Ryan, Barbara J, Villanueva, Augusto, Llovet, Josep M
Format:	Article
Language:	eng
Subjects:	Adenoma - genetics Adenoma - pathology Animals Biological and medical sciences Biotechnology Cancer Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - pathology Care and treatment DNA Transposable Elements - genetics Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation Genes, erbB-1 - genetics Genes, erbB-1 - physiology Genetic aspects Genetic Techniques Health. Pharmaceutical industry Hepatoma Humans Industrial applications and implications. Economical aspects Integrases - genetics Integrases - metabolism Liver - metabolism Liver cancer Liver Neoplasms, Experimental - genetics Liver Neoplasms, Experimental - metabolism Liver Neoplasms, Experimental - pathology Male Mice Mice, Transgenic Miscellaneous Mutagenesis Mutagenesis, Insertional - methods Physiological aspects Promoters (Genetics) Rodents Transposases - genetics Transposases - metabolism Transposons Ubiquitin-Conjugating Enzymes - genetics Ubiquitin-Conjugating Enzymes - physiology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!