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The vector competence of Phlebotomus perniciosus for Leishmania infantum zymodemes of Tunisia
Experimental infections of Phlebotomus (L.) perniciosus from a colony established in Madrid (Spain) carried out with the Leishmania (L.) infantum zymodemes MON-1, MON-24, and MON-80 isolated in Tunisia are reported here. Laboratory-reared female sand flies were experimentally fed via membrane feedin...
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Published in: | Parasitology research (1987) 2018-08, Vol.117 (8), p.2499-2506 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Experimental infections of
Phlebotomus (L.) perniciosus
from a colony established in Madrid (Spain) carried out with the
Leishmania (L.) infantum
zymodemes MON-1, MON-24, and MON-80 isolated in Tunisia are reported here. Laboratory-reared female sand flies were experimentally fed via membrane feeding device on a suspension of
L. infantum
promastigotes in defibrinated rabbit blood (10
7
/ml). Engorged females were dissected at progressive time points postfeeding to observe the intravectorial cycle of different
L. infantum
zymodemes. Development in the sand fly midgut of
L. infantum
parasites to the infective metacyclic promastigotes and monitoring the forward progression of parasites to finally reach the stomodeal valve (SV) of the sand fly were assessed. All tested
L. infantum
zymodemes developed properly in
P. perniciosus
. Experimental feeding with suspensions of promastigotes of all zymodemes led to very heavy late-stage infections. MON-24 and MON-80 zymodemes colonized the (SV) of
P. perniciosus
earlier than zymodeme MON-1, 2 and 4 days, respectively. Metacyclic promastigotes were observed in all experimental infections. The study shows for the first time that colonized
P. perniciosus
is able to acquire, retain, and develop in its midgut the zymodemes MON-24 and MON-80 isolated in Tunisia and highlights the putative role of this sand fly species in the transmission of such zymodemes to mammalian hosts in this country. The ability of experimentally infected sand fly species to transmit by bite such zymodemes needs to be assessed. |
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ISSN: | 0932-0113 1432-1955 |
DOI: | 10.1007/s00436-018-5939-y |