Incidence of Prunus necrotic ring spot virus on Malus domestica in India

In surveys of apple (Malus domestica) orchards in various parts of Himachal Pradesh, samples from trees showing necrotic symptoms on the leaves were collected and tested for detection of Prunus necrotic ringspot virus (PNRSV) initially by ELISA followed by RT-PCR using coat protein gene primers. Pos...

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Bibliographic Details
Published in:Journal of phytopathology 2008-06, Vol.156 (6), p.382-384
Main Authors: Chandel, V, Rana, T, Handa, A, Thakur, P.D, Hallan, V, Zaidi, A.A
Format: Article
Language:English
Subjects:
apple
apples
Biological and medical sciences
ELISA
enzyme-linked immunosorbent assay
Fundamental and applied biological sciences. Psychology
India
Malus
Malus domestica
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
PNRSV
Prunus necrotic ringspot virus
RT-PCR
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Summary:In surveys of apple (Malus domestica) orchards in various parts of Himachal Pradesh, samples from trees showing necrotic symptoms on the leaves were collected and tested for detection of Prunus necrotic ringspot virus (PNRSV) initially by ELISA followed by RT-PCR using coat protein gene primers. Positive results were obtained in samples from Kullu and Kalpa regions. The virus gene sequences showed 88-97% similarity to corresponding sequences of other PNRSV isolates deposited in the GenBank database using ncbi.nih.nlm.gov. Although the similarity was high, there were some distinct differences with the Spanish isolate. This is the first report of PNRSV in apple from India.
ISSN:0931-1785
1439-0434
DOI:10.1111/j.1439-0434.2007.01361.x