Heightened expression of HLA-DQB1 and HLA-DQB2 in pre-implantation biopsies predicts poor late kidney graft function

Background: Accurate pre-transplant prediction of late graft function remains an unmet need in kidney transplantation. The aim of this study was to evaluate HLA genes expression levels in pre-implantation biopsies (PIB) of deceased donor kidneys as markers for long-term graft outcome. Methods: HLA g...

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Bibliographic Details
Published in:Human immunology 2018-08, Vol.79 (8), p.594-601
Main Authors: Mine, Karina L., Tedesco-Silva, Hélio, Mourão, Tuíla B., Campos, Erika F., Salzedas, Larissa A., Aguiar, Bruna, Felipe, Claudia R., Medina-Pestana, Jose O., Gerbase-DeLima, Maria
Format: Article
Language:English
Subjects:
HLA expression
HLA-DQB1
HLA-DQB2
Kidney graft dysfunction
Pre-implantation biopsies
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Summary:Background: Accurate pre-transplant prediction of late graft function remains an unmet need in kidney transplantation. The aim of this study was to evaluate HLA genes expression levels in pre-implantation biopsies (PIB) of deceased donor kidneys as markers for long-term graft outcome. Methods: HLA genes expression analysis was initially performed using microarray data of 53 PIB, previously generated by our laboratory. The validation analysis was performed by real-time PCR in 116 PIB from an independent cohort. Results: The microarray data showed association between high expression levels of HLA class II genes, especially HLA-DQB1 and -DQB2, in kidneys from young (18 to 49-year-old) donors and poor (eGFR 
ISSN:0198-8859
1879-1166
DOI:10.1016/j.humimm.2018.05.002