Melissa officinalis L. ethanolic extract inhibits the growth of a lung cancer cell line by interfering with the cell cycle and inducing apoptosis

Melissa officinalis is a plant from the family Lamiaceae, native in Europe particularly in the Mediterranean region. Given our interest in identifying extracts and compounds capable of inhibiting tumor cell growth, and given the antioxidant content and the high consumption of Melissa officinalis in...

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Bibliographic Details
Published in:Food & function 2018-06, Vol.9 (6), p.3134-3142
Main Authors: Magalhães, D. B, Castro, I, Lopes-Rodrigues, V, Pereira, J. M, Barros, L, Ferreira, I. C. F. R, Xavier, C. P. R, Vasconcelos, M. H
Format: Article
Language:English
Subjects:
Antineoplastic Agents, Phytogenic - pharmacology
Antioxidants
Apoptosis
Apoptosis - drug effects
Biotechnology
Cancer
Caspase
Caspase-3
Cell cycle
Cell Cycle - drug effects
Cell growth
Cell Line, Tumor
Cell Proliferation - drug effects
Humans
Lung cancer
Lung Neoplasms - drug therapy
Lung Neoplasms - physiopathology
Melissa - chemistry
Melissa officinalis
Natural products
p53 Protein
Phenols
Plant extracts
Plant Extracts - pharmacology
Rosmarinic acid
Tumor cell lines
Tumors
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Summary:Melissa officinalis is a plant from the family Lamiaceae, native in Europe particularly in the Mediterranean region. Given our interest in identifying extracts and compounds capable of inhibiting tumor cell growth, and given the antioxidant content and the high consumption of Melissa officinalis in Portugal, this study aimed to test the tumor cell growth inhibitory activity of five different extracts of this plant (aqueous, methanolic, ethanolic, hydromethanolic and hydroethanolic) in three human tumor cell lines: MCF-7, AGS and NCI-H460. All extracts decreased cell growth in all cell lines in a concentration-dependent manner. The ethanolic extract was the most potent one, presenting a GI 50 concentration of approximately 100.9 μg mL −1 in the NCI-H460 lung cancer cells. This extract was characterized by LC-DAD-ESI/MS regarding its phenolic composition, revealing rosmarinic acid as the most abundant compound. The GI 75 concentration of this extract affected the cell cycle profile of these cells. In addition, both the GI 50 and the GI 75 concentrations of the extract induced cellular apoptosis. Moreover, treatment of NCI-H460 cells with this extract caused a decrease in pro-caspase 3 and an increase in p53 levels. This study emphasizes the relevance of the study of natural products as inhibitors of tumor cell growth. Melissa officinalis is a plant from the family Lamiaceae, native in Europe particularly in the Mediterranean region.
ISSN:2042-6496
2042-650X
DOI:10.1039/c8fo00446c