Development of an opsonophagocytic killing assay for group a streptococcus

•OPKA developed for a number of clinically relevant Group A Streptococcus.•Homologous M-protein antisera shown to kill GAS emm-types 1, 6 and 12.•Killing inhibited by homologous but not heterologous antigens proving specificity.•GAS OPKA is precise; with intra and inter-assay coefficients of variati...

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Bibliographic Details
Published in:Vaccine 2018-06, Vol.36 (26), p.3756-3763
Main Authors: Jones, Scott, Moreland, Nicole J., Zancolli, Marta, Raynes, Jeremy, Loh, Jacelyn M.S., Smeesters, Pierre R., Sriskandan, Shiranee, Carapetis, Jonathan R., Fraser, John D., Goldblatt, David
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - blood
Antigens
Antisera
Assaying
Baby rabbit complement
Cell Line
Complement
Complement System Proteins - immunology
Dimethylformamide
Functional assay
GAS vaccines
Gram-positive bacteria
HL-60 cells
Homology
Humans
Immunoassay - methods
Immunoassay - standards
Immunoglobulins
Leukemia
Leukocytes (neutrophilic)
Linearity
Microbial Viability
Neutrophils - immunology
Opsonin Proteins - blood
Opsonophagocytic killing assay
Phagocytosis
Promyeloid leukemia
Proteins
Rabbits
Sensitivity and Specificity
Streptococcal Infections - immunology
Streptococcus
Streptococcus infections
Streptococcus pyogenes
Streptococcus pyogenes - immunology
Streptococcus pyogenes - physiology
Vaccine development
Vaccines
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Description
Summary:•OPKA developed for a number of clinically relevant Group A Streptococcus.•Homologous M-protein antisera shown to kill GAS emm-types 1, 6 and 12.•Killing inhibited by homologous but not heterologous antigens proving specificity.•GAS OPKA is precise; with intra and inter-assay coefficients of variation
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2018.05.056