Repair potential of nonsurgically delivered induced pluripotent stem cell‐derived chondrocytes in a rat osteochondral defect model

Human induced pluripotent stem cells (hiPSCs) are thought to be an alternative cell source for future regenerative medicine. hiPSCs may allow unlimited production of cell types that have low turnover rates and are difficult to obtain such as autologous chondrocytes. In this study, we generated hiPSC...

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Bibliographic Details
Published in:Journal of tissue engineering and regenerative medicine 2018-08, Vol.12 (8), p.1843-1855
Main Authors: Rim, Yeri Alice, Nam, Yoojun, Park, Narae, Lee, Jennifer, Park, Sung‐hwan, Ju, Ji Hyeon
Format: Article
Language:English
Subjects:
Autografts
Biocompatibility
Cartilage
chondrocyte
Chondrocytes
chondrogenesis
Collagen (type II)
Defects
differentiation
Extracellular matrix
induced pluripotent stem cell
intra‐articular injection
Knee
osteoarthritis
Pellets
Pluripotency
Proteins
Recovery
Regeneration (physiology)
Regenerative medicine
Stem cell transplantation
Stem cells
Tissue engineering
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Summary:Human induced pluripotent stem cells (hiPSCs) are thought to be an alternative cell source for future regenerative medicine. hiPSCs may allow unlimited production of cell types that have low turnover rates and are difficult to obtain such as autologous chondrocytes. In this study, we generated hiPSC‐derived chondrogenic pellets, and chondrocytes were isolated. To confirm the curative effects, chondrogenic pellets and isolated chondrocytes were transplanted into rat joints with osteochondral defects. Isolated hiPSC‐derived chondrocytes were delivered in the defect by a single intra‐articular injection. The generated hiPSC‐derived chondrogenic pellets had increased chondrogenic marker expression and accumulated extracellular matrix proteins. Chondrocytes were successfully isolated from the pellets. Alcian blue staining and collagen type II were detected in the cells. Chondrogenic marker expression was also increased in the isolated cells. Transplanted chondrogenic pellets and chondrocytes both had curative effects in the osteochondral defect rat model. Detection of human proteins in the joints proved that the cells were successfully delivered into the defect. Chondrogenic pellets or chondrocytes generated from hiPSCs have potential as regenerative medicine for cartilage recovery or regeneration. Chondrocytes isolated from hiPSC‐derived chondrogenic pellets had curative effects in damaged cartilage. Injectable hiPSC‐derived chondrocytes show the possibility of noninvasive delivery of regenerative medicine for cartilage recovery.
ISSN:1932-6254
1932-7005
DOI:10.1002/term.2705