Precisely controlled delivery of magnesium ions thru sponge-like monodisperse PLGA/nano-MgO-alginate core-shell microsphere device to enable in-situ bone regeneration

A range of magnesium ions (Mg2+) used has demonstrated osteogenic tendency in vitro. Hence, we propose to actualize this concept by designing a new system to precisely control the Mg2+ delivery at a particular concentration in vivo in order to effectively stimulate in-situ bone regeneration. To achi...

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Bibliographic Details
Published in:Biomaterials 2018-08, Vol.174, p.1-16
Main Authors: Lin, Zhengjie, Wu, Jun, Qiao, Wei, Zhao, Ying, Wong, Karen H.M., Chu, Paul K., Bian, Liming, Wu, Shuilin, Zheng, Yufeng, Cheung, Kenneth M.C., Leung, Frankie, Yeung, Kelvin W.K.
Format: Article
Language:English
Subjects:
Biocompatibility
Bone regeneration
Core-shell microspheres
Microfluidic capillary device
Precisely controlled magnesium ion release
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Summary:A range of magnesium ions (Mg2+) used has demonstrated osteogenic tendency in vitro. Hence, we propose to actualize this concept by designing a new system to precisely control the Mg2+ delivery at a particular concentration in vivo in order to effectively stimulate in-situ bone regeneration. To achieve this objective, a monodisperse core-shell microsphere delivery system comprising of poly (lactic-co-glycolic acid) (PLGA) biopolymer, alginate hydrogel, and magnesium oxide nano-particles has been designed by using customized microfluidic capillary device. The PLGA-MgO sponge-like spherical core works as a reservoir of Mg2+ while the alginate shell serves as physical barrier to control the outflow of Mg2+ at ∼50 ppm accurately for 2 weeks via its adjustable surface micro-porous network. With the aid of controlled release of Mg2+, the new core-shell microsphere system can effectively enhance osteoblastic activity in vitro and stimulate in-situ bone regeneration in vivo in terms of total bone volume, bone mineral density (BMD), and trabecular thickness after operation. Interestingly, the Young's moduli of formed bone on the core-shell microsphere group have been restored to ∼96% of that of the surrounding matured bone. These findings indicate that the concept of precisely controlled release of Mg2+ may potentially apply for in-situ bone regeneration clinically. [Display omitted]
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2018.05.011