Clavanin A-bioconjugated Fe3O4/Silane core-shell nanoparticles for thermal ablation of bacterial biofilms

[Display omitted] •We developed a simple strategy that employs a bifunctional clavanin A/Fe3O4 hybrid nanosystem.•CVC tubing was modified by immobilization of the nanosystem.•The proposed tube coating had excellent antimicrobial activity.•The obtained results are very similar to those of commercial...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2018-09, Vol.169, p.72-81
Main Authors: Ribeiro, Kalline L., Frías, Isaac A.M., Franco, Octavio L., Dias, Simoni C., Sousa-Junior, Ailton A., Silva, Osmar N., Bakuzis, Andris F., Oliveira, Maria D.L., Andrade, Cesar A.S.
Format: Article
Language:English
Subjects:
Antimicrobial peptides
Biofilm
Catheter
Clavanin A
Hyperthermia
Online Access:Get full text
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Summary:[Display omitted] •We developed a simple strategy that employs a bifunctional clavanin A/Fe3O4 hybrid nanosystem.•CVC tubing was modified by immobilization of the nanosystem.•The proposed tube coating had excellent antimicrobial activity.•The obtained results are very similar to those of commercial antibiotic. The use of central venous catheters (CVC) is highly associated with nosocomial blood infections and its use largely requires a systematic assessment of benefits and risks. Bacterial contamination of these tubes is frequent and may result in development of microbial consortia also known as biofilm. The woven nature of biofilm provides a practical defense against antimicrobial agents, facilitating bacterial dissemination through the patient’s body and development of antimicrobial resistance. In this work, the authors describe the modification of CVC tubing by immobilizing Fe3O4-aminosilane core-shell nanoparticles functionalized with antimicrobial peptide clavanin A (clavA) as an antimicrobial prophylactic towards Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. Its anti-biofilm-attachment characteristic relies in clavA natural activity to disrupt the bacterial lipidic membrane. The aminosilane shell prevents iron leaching, which is an important nutrient for bacterial growth. Fe3O4-clavA-modified CVCs showed to decrease Gram-negative bacteria attachment up to 90% when compared to control clean CVC. Additionally, when hyperthermal treatment is triggered for 5 min at 80 °C in a tubing that already presents bacterial biofilm (CVC-BF), the viability of attached bacteria reduces up to 88%, providing an efficient solution to avoid changing catheter.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2018.04.055