Detection of clastogenic and aneugenic damage in newborn rats

The last 25 years have seen an ever‐growing use of the erythrocyte micronucleus test for measuring damage to mammalian chromosomes in vivo. In addition, staining micronuclei with antikinetochore antibodies from CREST serum discriminates aneugenic from clastogenic damage. The use of the micronucleus...

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Bibliographic Details
Published in:Environmental and molecular mutagenesis 2006-06, Vol.47 (5), p.320-324
Main Authors: Udroiu, Ion, Ieradi, Luisa Anna, Cristaldi, Mauro, Tanzarella, Caterina
Format: Article
Language:English
Subjects:
Aneugens - toxicity
Animals
Animals, Newborn
Biological and medical sciences
CREST
Erythrocytes - drug effects
Erythrocytes - radiation effects
Female
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
liver
Liver - drug effects
Liver - radiation effects
Male
Medical sciences
micronuclei
Micronuclei, Chromosome-Defective - chemically induced
Micronuclei, Chromosome-Defective - radiation effects
Micronucleus Tests - methods
Mutagens - toxicity
newborn rats
peripheral blood
Rats
Rats, Wistar
Toxicology
Vinblastine - toxicity
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Summary:The last 25 years have seen an ever‐growing use of the erythrocyte micronucleus test for measuring damage to mammalian chromosomes in vivo. In addition, staining micronuclei with antikinetochore antibodies from CREST serum discriminates aneugenic from clastogenic damage. The use of the micronucleus test in rats, however, has been problematic because the spleen of adult rats efficiently removes micronucleated erythrocytes from the blood. In the present study, we have treated 5‐day‐old rats with either X‐rays (a clastogen) or vinblastine (an aneugen) and measured micronuclei in erythrocytes from the blood and liver. Each treatment increased the frequency of micronuclei in both tissues, with the percentages of CREST‐staining micronuclei reflecting the mechanism of micronucleus induction by the two agents. The results indicate that performing the micronucleus assay in the liver and peripheral blood of 5‐day‐old rats may be a useful approach for detecting the in vivo genotoxicity of chemical and physical agents. Environ. Mol. Mutagen., 2006. © 2006 Wiley‐Liss, Inc.
ISSN:0893-6692
1098-2280
DOI:10.1002/em.20209