Development of an Immunosensor Based on Surface Plasmon Resonance for Simultaneous Residue Analysis of Three Pesticides —Boscalid, Clothianidin, and Nitenpyram— in Vegetables

A simultaneous immunosensor based on surface plasmon resonance (SPR) was developed for determination of 3 pesticides —boscalid, clothianidin and nitenpyram— instead of the direct competitive enzyme-linked immunosorbent assays (dcELISAs) widely used as individual determination methods. Carboxy groups...

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Bibliographic Details
Published in:Analytical Sciences 2018/05/10, Vol.34(5), pp.533-539
Main Authors: HIRAKAWA, Yuki, YAMASAKI, Tomomi, HARADA, Ayako, IWASA, Seiji, NARITA, Hiroshi, MIYAKE, Shiro
Format: Article
Language:English
Subjects:
Analytical Chemistry
antibody
Bovine serum albumin
Chemistry
fungicide
immunoassay
Immunoassays
Immunosensors
Insecticide
Monoclonal antibodies
Pesticide residues
Pesticides
Resonance
sensor
Serum albumin
Surface plasmon resonance
Vegetables
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Summary:A simultaneous immunosensor based on surface plasmon resonance (SPR) was developed for determination of 3 pesticides —boscalid, clothianidin and nitenpyram— instead of the direct competitive enzyme-linked immunosorbent assays (dcELISAs) widely used as individual determination methods. Carboxy groups that introduced compounds to their pesticides were designed, and conjugates of them and bovine serum albumin were immobilized onto separate channels of the same sensor chip. When a mixture of 3 monoclonal antibodies reacted to each pesticide, and 3 pesticides were injected into the SPR immunosensor, each channel showed specific reactivity at 15 – 93 ng mL−1 for boscalid, 6.7 – 27 ng mL−1 for clothianidin, and 7.3 – 62 ng mL−1 for nitenpyram. Recovery tests using vegetables spiked with a mixture of 3 pesticides showed good results: 75 – 90%, 88 – 104%, and 72 – 105%, respectively, with a high correlation to results of the dcELISAs. The SPR immunosensor would be useful for the determination of pesticide residues in vegetables.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.17P487