HPV-16 viral load in oropharyngeal squamous cell carcinoma using digital PCR

Conclusions: We did not identify any strong associations between HPV-16 viral load and any of the clinical or lifestyle factors. Objective: The epidemiology of oropharyngeal SCC is changing, with an increasing proportion of HPV-positive cases seen in the last decade. It is known that a high viral lo...

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Bibliographic Details
Published in:Acta oto-laryngologica 2018-09, Vol.138 (9), p.843-847
Main Authors: Antonsson, Annika, Knight, Lani, Panizza, Benedict J., Porceddu, Sandro V., Emmett, Sarah, Whiteman, David C.
Format: Article
Language:English
Subjects:
Adult
Aged
Carcinoma, Squamous Cell - virology
Female
HPV-16
Human papilloma virus
Human papillomavirus 16 - genetics
Human papillomavirus 16 - isolation & purification
Humans
Life Style
lifestyle factors
Male
Middle Aged
Oropharyngeal Neoplasms - virology
oropharyngeal SCC
Papillomavirus Infections - complications
Papillomavirus Infections - virology
Polymerase Chain Reaction
Viral Load
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Summary:Conclusions: We did not identify any strong associations between HPV-16 viral load and any of the clinical or lifestyle factors. Objective: The epidemiology of oropharyngeal SCC is changing, with an increasing proportion of HPV-positive cases seen in the last decade. It is known that a high viral load is linked to the development of cervical cancer, the relation between viral load and oropharyngeal SCC is less clear. We sought to determine HPV-16 viral load in HPV-positive oropharyngeal SCCs using highly sensitive digital PCR and to identify clinical and lifestyle factors associated with viral load. Subjects and methods: We analysed 45 HPV-16 positive oropharyngeal SCCs diagnosed between 2013 and 2015. All patients completed a lifestyle questionnaire and clinical data were extracted from medical charts. Viral load was determined using digital PCR assays for HPV-L1 and RNAseP. Results: We found large variations in HPV-16 viral load from 1 to 930 copies per cell (median 34 copies per cell).
ISSN:0001-6489
1651-2251
DOI:10.1080/00016489.2018.1461239