High-Fat Diet Consumption Induces Microbiota Dysbiosis and Intestinal Inflammation in Zebrafish

Energy-dense foods and overnutrition represent major starting points altering lipid metabolism, systemic inflammation and gut microbiota. The aim of this work was to investigate the effects of a high-fat diet (HFD) over a period of 25 days on intestinal microbiota and inflammation in zebrafish. Micr...

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Bibliographic Details
Published in:Microbial ecology 2018-11, Vol.76 (4), p.1089-1101
Main Authors: Arias-Jayo, Nerea, Abecia, Leticia, Alonso-Sáez, Laura, Ramirez-Garcia, Andoni, Rodriguez, Alfonso, Pardo, Miguel A.
Format: Article
Language:English
Subjects:
Animals
Anti-Infective Agents - metabolism
Bacteria - classification
Bacteria - drug effects
Bacteria - genetics
Biomedical and Life Sciences
Community composition
Composition
Danio rerio
Diet
Diet, High-Fat - adverse effects
Disease Models, Animal
DNA
Dysbacteriosis
Dysbiosis - chemically induced
Ecology
Freshwater fishes
Gastrointestinal Microbiome - drug effects
Gastrointestinal Microbiome - genetics
Gene expression
Gene Expression Regulation, Bacterial
Gene sequencing
Genes
Genes, Bacterial
Geoecology/Natural Processes
Goblet Cells - metabolism
High fat diet
HOST MICROBE INTERACTIONS
Immune System
Inflammation
Intestinal microflora
Intestine
Intestines
Intestines - microbiology
Intestines - pathology
Life Sciences
Lipid metabolism
Lipids
Metabolism
Microbial Ecology
Microbiology
Microbiota
Microorganisms
Mucin
Mucins - metabolism
Nature Conservation
Nucleotide sequence
Overnutrition
PCR
Receptors
Relative abundance
RNA, Ribosomal, 16S - genetics
rRNA 16S
Water Quality/Water Pollution
Zebrafish
Zebrafish - immunology
Zebrafish - microbiology
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Summary:Energy-dense foods and overnutrition represent major starting points altering lipid metabolism, systemic inflammation and gut microbiota. The aim of this work was to investigate the effects of a high-fat diet (HFD) over a period of 25 days on intestinal microbiota and inflammation in zebrafish. Microbial composition of HFD-fed animals was analysed and compared to controls by 16S rRNA sequencing and quantitative PCR. The expression level on several genes related to inflammation was tested. Furthermore, microscopic assessment of the intestine was performed in both conditions. The consumption of the HFD resulted in microbial dysbiosis, characterised by an increase in the relative abundance of the phylum Bacteroidetes. Moreover, an emerging intestinal inflammation via NF-κβ activation was confirmed by the overexpression of several genes related to signalling receptors, antimicrobial metabolism and the inflammatory cascade. The intestinal barrier was also damaged, with an increase of goblet cell mucin production. This is the first study performed in zebrafish which suggests that the consumption of a diet enriched with 10% fat changes the intestinal microbial community composition, which was correlated with low-grade inflammation.
ISSN:0095-3628
1432-184X
DOI:10.1007/s00248-018-1198-9