Magnetic nanoparticle decorated graphene based electrochemical nanobiosensor for H2O2 sensing using HRP

[Display omitted] •MNP, RGO and MRGO were prepared and characterized using XRD, FTIR and TEM.•MNP, RGO and MRGO were used for electrochemical sensing of H2O2 with HRP on platinum electrode.•MRGO showed highest sensitivity due to synergetic effect of the RGO and MNP. To utilize synergetic effect of g...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2018-07, Vol.167, p.425-431
Main Authors: Waifalkar, P.P., Chougale, A.D., Kollu, P., Patil, P.S., Patil, P.B.
Format: Article
Language:English
Subjects:
Electrochemical biosensor
H2O2
Horseradish peroxidase
Magnetic nanoparticle decorated graphene
MNP
RGO
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Summary:[Display omitted] •MNP, RGO and MRGO were prepared and characterized using XRD, FTIR and TEM.•MNP, RGO and MRGO were used for electrochemical sensing of H2O2 with HRP on platinum electrode.•MRGO showed highest sensitivity due to synergetic effect of the RGO and MNP. To utilize synergetic effect of graphene’s higher conductivity and magnetic nanoparticles biocompatibility, an electrochemical nanobiosensor is constructed based on magnetic nanoparticle decorated graphene (MRGO) using Horseradish peroxidase (HRP) for H2O2 sensing. Sensors based on magnetic nanoparticles (MNP) and reduced graphene oxide (RGO) are studied for comparison. MNP, RGO and MRGO were characterized by X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). XRD studies have confirmed successful synthesis of Fe3O4 MNPs, RGO and MRGO. TEM micrographs revealed uniform decoration of MNPs on graphene. FTIR confirmed the immobilization of HRP on MNP, RGO and MRGO. The MRGO based sensor exhibited higher sensitivity (48.08 μA μM−1 cm−2) compared to MNP (39.08 μA μM−1 cm−2) and RGO (41.08 μA μM−1 cm−2) based biosensors.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2018.04.042