A pilot study using a novel pyrotechnically driven prototype applicator for epidermal powder immunization in piglets

[Display omitted] Epidermal powder immunization (EPI) is an alternative technique to the classical immunization route using needle and syringe. In this work, we present the results of an in vivo pilot study in piglets using a dried influenza model vaccine which was applied by EPI using a novel pyrot...

Full description

Saved in:
Bibliographic Details
Published in:International journal of pharmaceutics 2018-07, Vol.545 (1-2), p.215-228
Main Authors: Engert, Julia, Anamur, Cihad, Engelke, Laura, Fellner, Christian, Lell, Peter, Henke, Stefan, Stadler, Julia, Zöls, Susanne, Ritzmann, Mathias, Winter, Gerhard
Format: Article
Language:English
Subjects:
antibody response
Epidermal powder immunization
H1N1 vaccine
in vivo
pig
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] Epidermal powder immunization (EPI) is an alternative technique to the classical immunization route using needle and syringe. In this work, we present the results of an in vivo pilot study in piglets using a dried influenza model vaccine which was applied by EPI using a novel pyrotechnically driven applicator. A liquid influenza vaccine (Pandemrix®) was first concentrated by tangential flow filtration and hemagglutinin content was determined by RP-HPLC. The liquid formulation was then transformed into a dry powder by collapse freeze-drying and subsequent cryo-milling. The vaccine powder was attached to a membrane of a novel pyrotechnical applicator using oily adjuvant components. Upon actuation of the applicator, particles were accelerated to high speed as determined by a high-speed camera setup. Piglets were immunized twice using either the novel pyrotechnical applicator or classical intramuscular injection. Blood samples of the animals were collected at various time points and analyzed by enzyme-linked immunosorbent assay. Our pilot study shows that acceleration of a dried vaccine powder to supersonic speed using the pyrotechnical applicator is possible and that the speed and impact of the particles is sufficient to breach the stratum corneum of piglet skin. Importantly, the administration of the dry vaccine powder resulted in measurable anti-H1N1 antibody titres in vivo.
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2018.04.039