Aqueous and Ethanol Extracts of Daylily Flower (Hemerocallis fulva L.) Protect HUVE Cells Against High Glucose

The content of several phenolic acids and flavonoids in aqueous extract (AE) and ethanol extract (EE) of daylily flower (Hemerocallis fulva L.) was analyzed. The effects of AE or EE at 0.5%, 1%, or 2% in HUVE cells against high glucose‐induced cell death, oxidative, and inflammatory damage were exam...

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Published in:Journal of food science 2018-05, Vol.83 (5), p.1463-1469
Main Authors: Wu, Wen‐Tzu, Mong, Mei‐chin, Yang, Ya‐chen, Wang, Zhi‐hong, Yin, Mei‐chin
Format: Article
Language:English
Subjects:
Acids
Apoptosis
Caspase
Catalase
Cell death
Diabetes mellitus
Ethanol
Flavonoids
Gene expression
Glucose
Glutathione
Glutathione peroxidase
Glutathione reductase
Hemerocallis fulva
Hemerocallis fulva L
high glucose
HUVE cell
Inflammation
Interleukins
Peroxidase
Phenolic acids
Phenols
phytochemicals
Prostaglandin E2
Prostaglandin endoperoxide synthase
Reactive oxygen species
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Summary:The content of several phenolic acids and flavonoids in aqueous extract (AE) and ethanol extract (EE) of daylily flower (Hemerocallis fulva L.) was analyzed. The effects of AE or EE at 0.5%, 1%, or 2% in HUVE cells against high glucose‐induced cell death, oxidative, and inflammatory damage were examined. Results showed that seven phenolic acids and seven flavonoids could be detected in AE or EE, in the range of 29 to 205 and 41 to 273 mg/100 g, respectively. Compared with the control groups, high glucose raised the activity of caspase‐3 and caspase‐8; suppressed Bcl‐2 mRNA expression and increased Bax mRNA expression; and induced HUVE cells apoptosis. The pretreatments from AE or EE at 1% or 2% reduced caspase‐3 activity and Bax mRNA expression, and enhanced cell viability. High glucose decreased glutathione content; stimulated the production of reactive oxygen species, interleukin‐6, tumor necrosis factor‐alpha, and prostaglandin E2; raised the activity of cyclooxygenase‐2 and nuclear factor kappa B p50/65 binding; and reduced the activity of glutathione peroxidase, glutathione reductase, and catalase in HUVE cells. AE pretreatments at 1% and 2% reversed these changes. These novel findings suggested that daylily flower was rich in phytochemicals, and could be viewed as a potent functional food against diabetes.
ISSN:0022-1147
1750-3841
DOI:10.1111/1750-3841.14137