Effects of resveratrol in inflammatory arthritis

Nuclear factor kappa B (NF-kappaB), is a pivotal transcription factor involved in the activation of the TNF-alpha and IL-1beta genes. Activation of NF-kappaB in synovial cells is a feature seen in arthritis patients. Resveratrol, a polyphenolic, natural phytoalexin found with particularly high level...

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Bibliographic Details
Published in:Inflammation 2007-04, Vol.30 (1-2), p.1-6
Main Authors: Elmali, N, Baysal, O, Harma, A, Esenkaya, I, Mizrak, B
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents, Non-Steroidal - administration & dosage
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Anti-Inflammatory Agents, Non-Steroidal - therapeutic use
Arthritis, Experimental - chemically induced
Arthritis, Experimental - pathology
Arthritis, Experimental - prevention & control
Arthritis, Rheumatoid - chemically induced
Arthritis, Rheumatoid - pathology
Arthritis, Rheumatoid - prevention & control
Cartilage - chemistry
Cartilage - drug effects
Cartilage - pathology
Female
Injections, Intra-Articular
Knee Joint - chemistry
Knee Joint - drug effects
Knee Joint - pathology
Lipopolysaccharides
Proteoglycans - analysis
Rabbits
Stilbenes - administration & dosage
Stilbenes - pharmacology
Stilbenes - therapeutic use
Synovial Membrane - drug effects
Synovial Membrane - pathology
Vitaceae
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Summary:Nuclear factor kappa B (NF-kappaB), is a pivotal transcription factor involved in the activation of the TNF-alpha and IL-1beta genes. Activation of NF-kappaB in synovial cells is a feature seen in arthritis patients. Resveratrol, a polyphenolic, natural phytoalexin found with particularly high levels in grape skin and red wine is potent and specific inhibitor of TNF-alpha and IL-1beta induced NF-kappaB activation. We aimed to determine the in vivo effects of intra-articular injections of resveratrol on cartilage and synovium in an experimental rabbit inflammatory arthritis model. Arthritis was induced by intra-articular injection of three times of 50 mug lipopolysaccharide (LPS) at day 0, 4 and 8 at 4-day intervals into the knee joints of rabbits. To the test group, 10 muMol/kg resveratrol in the DMSO was injected in the knees at day 0 and then it was continued once daily for 2 weeks. To the control group the same time and amount of DMSO was injected the knees of rabbits. All rabbits were killed 1 week after the last injection and cartilage tissue and synovium were evaluated with semiquantitative scoring histologically. According to control group in the resveratrol group, significantly decreased cartilage destruction was determined by H&E staining (p = 0.04). Loss of matrix proteoglycan content in the cartilage was much lower, as determined by safranin O staining (p = 0.03). We also observed marked synovial inflammation after intra-articular injection to control knees, but not in the resveratrol treated group knees (p = 0.01). This study suggests that intra-articular injection of resveratrol may protect cartilage against the development of experimentally induced IA.
ISSN:0360-3997
1573-2576
DOI:10.1007/s10753-006-9012-0