Anhydrous polymer‐based coating with sustainable controlled release functionality for facile, efficacious impregnation, and delivery of antimicrobial peptides

Anhydrous polymers are actively explored as alternative materials to overcome limitations of conventional hydrogel‐based antibacterial coating. However, the requirement for strong organic solvent in polymerization reactions often necessitates extra protection steps for encapsulation of target biomol...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and bioengineering 2018-08, Vol.115 (8), p.2000-2012
Main Authors: Lim, Kaiyang, Saravanan, Rathi, Chong, Kelvin K. L., Goh, Sharon H. M., Chua, Ray R. Y., Tambyah, Paul A., Chang, Matthew W., Kline, Kimberly A., Leong, Susanna S. J.
Format: Article
Language:English
Subjects:
anhydrous polymer coating
Antibacterial materials
Antimicrobial peptides
Biocompatibility
Biomolecules
Catheters
Coating effects
Coatings
Conformation
Controlled release
Diffusion barriers
Diffusion layers
Effectiveness
Encapsulation
Hydrogels
Kinetics
Peptides
Phosphocholine
Polycaprolactone
Polymerization
Polymers
Protective coatings
Reaction kinetics
Silicones
Solvation
Trifluoroethanol
trifluoroethanol solvent
urinary catheter
Viability
Wounding
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Anhydrous polymers are actively explored as alternative materials to overcome limitations of conventional hydrogel‐based antibacterial coating. However, the requirement for strong organic solvent in polymerization reactions often necessitates extra protection steps for encapsulation of target biomolecules, lowering encapsulation efficiency, and increasing process complexity. This study reports a novel coating strategy that allows direct solvation and encapsulation of antimicrobial peptides (HHC36) into anhydrous polycaprolactone (PCL) polymer‐based dual layer coating. A thin 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine (POPC) film is layered onto the peptide‐impregnated PCL as a diffusion barrier, to modulate and enhance release kinetics. The impregnated peptides are eventually released in a controlled fashion. The use of 2,2,2‐trifluoroethanol (TFE), as polymerization and solvation medium, induces the impregnated peptides to adopt highly stable turned conformation, conserving peptide integrity, and functionality during both encapsulation and subsequent release processes. The dual layer coating showed sustained antibacterial functionality, lasting for 14 days. In vivo assessment using an experimental mouse wounding model demonstrated good biocompatibility and significant antimicrobial efficacy of the coating under physiological conditions. The coating was translated onto silicone urinary catheters and showed promising antibacterial efficacy, even outperforming commercial silver‐based Dover cather. This anhydrous polymer‐based platform holds immense potential as an effective antibacterial coating to prevent clinical device‐associated infections. The simplicity of the coating process enhances its industrial viability. Novel anhydrous polymer‐based antimicrobial peptide‐impregnated coating, consisting of poly(caprolactone) and 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine, is developed. Use of helix stabilizing 2,2,2‐trifluoroethanol as polymerization medium and solvent enables direct impregnation of antimicrobial peptide into the polymer, without requiring additional protection steps, enhancing loading efficacy. The coating demonstrated an ideal peptide release profile with prolonged antimicrobial and anti‐biofilm potency. Easy application onto commercial urinary catheter demonstrated excellent translatability of reported coating.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.26713