TNFα-Induced Expression of Transport Protein Genes in HUVEC Cells Is Associated with Enhanced Expression of Transcription Factor Genes RELB and NFKB2 of the Non-Canonical NF-κB Pathway

Endothelial HUVEC cells used as an in vitro model of the endothelial monolayer in placental barrier were activated by TNFα in a dose of 2 ng/ml for 24 h. Significant changes in the expression of genes of the SLC family transport protein were observed: an increase in the expression of SLC7A2 , SLC12A...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2018-04, Vol.164 (6), p.757-761
Main Authors: Knyazev, E. N., Mal’tseva, D. V., Zacharyants, A. A., Zakharova, G. S., Zhidkova, O. V., Poloznikov, A. A.
Format: Article
Language:English
Subjects:
Binding sites
Biomedical and Life Sciences
Biomedicine
Cell Biology
Gene expression
Internal Medicine
Laboratory Medicine
Magnesium
NF-κB protein
Pathology
Placenta
Protein transport
Protons
RelB protein
Sodium
Tumor necrosis factor-α
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Summary:Endothelial HUVEC cells used as an in vitro model of the endothelial monolayer in placental barrier were activated by TNFα in a dose of 2 ng/ml for 24 h. Significant changes in the expression of genes of the SLC family transport protein were observed: an increase in the expression of SLC7A2 , SLC12A2 , SLC9B2 , SLC25A37 , SLC16A9 , and SLC41A2 and a decrease in the expression of SLC40A1 . These transporters participate in the transport of iron, magnesium, sodium, potassium, and chloride ions, protons, and amino acids. It was also found that SLC7A2 , SLC12A2 , SLC9B2 , SLC25A37 , and SLC41A2 genes have binding sites for transcriptional factor RelB that together with NFKB2 is the main effector of the non-canonical NF-κB pathway. The expression of RELB and NFKB2 genes was also significantly enhanced in TNFα-activated HUVEC cells, which can attest to the important role of the non-canonical NF-κB pathway in the regulation of gene expression of transport proteins in response to TNFα stimulation.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-018-4074-1