Docosahexaenoic acid reversed atherosclerotic changes in human endothelial cells induced by palmitic acid in vitro

Abnormal activity of atherosclerotic endothelial cells paving luminal surface of blood vessels has been described in many diseases. It has been reported that natural polyunsaturated fatty acids such as docosahexaenoic acid exert therapeutic effects in atherosclerotic condition. Human umbilical vein...

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Bibliographic Details
Published in:Cell biochemistry and function 2018-06, Vol.36 (4), p.203-211
Main Authors: Karbasforush, Saeede, Nourazarian, Alireza, Darabi, Masoud, Rahbarghazi, Reza, Khaki‐Khatibi, Fatemeh, Biray Avci, Çıgır, Salimi, Leila, Goker Bagca, Bakiye, Novin Bahador, Tanaz, Rezabakhsh, Aysa, Khaksar, Majid
Format: Article
Language:English
Subjects:
Apoptosis
Arteriosclerosis
Atherosclerosis
atherosclerotic changes
Attenuation
Baby foods
Blood coagulation
Blood pressure
Blood vessels
cell survival
Clotting
Coagulation
Cytokines
Docosahexaenoic acid
Endothelial cells
Exposure
Fatty acids
Gene expression
Genes
HUVECs
Inflammation
Interleukins
Nitric oxide
Nitric-oxide synthase
Palmitic acid
Polymerase chain reaction
Polyunsaturated fatty acids
Prostaglandin E2
Reagents
Transcription
Umbilical vein
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Summary:Abnormal activity of atherosclerotic endothelial cells paving luminal surface of blood vessels has been described in many diseases. It has been reported that natural polyunsaturated fatty acids such as docosahexaenoic acid exert therapeutic effects in atherosclerotic condition. Human umbilical vein endothelial cells were treated with 1mM palmitic acid for 48 hours and exposed to 40μM docosahexaenoic acid for the next 24 hours. Real‐time polymerase chain reaction analysis was used to measure the expression of PTX3, iNOS, and eNOS. The level of nitric oxide was detected by Griess reagent. The transcription level of genes participating in coagulation and blood pressure was studied by polymerase chain reaction array. Docosahexaenoic acid improved the survival rate by reducing apoptosis rate (P 
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.3332