Structural predication and antigenic analysis of Toxoplasma gondii ROP20

Toxoplasma gondii infects almost all the warm-blooded animals. ROP20 protein is expressed in the rhoptry of Toxoplasma gondii . In this study, the secondary structure of ROP20 was analyzed using SMART software. We constructed and analyzed the 3D model of ROP20 protein using SWISS-MODEL online proced...

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Bibliographic Details
Published in:Acta parasitologica 2018-06, Vol.63 (2), p.244-251
Main Authors: Zhou, Jian, Wang, Wanchun, Song, Pengxia, Wang, Lin, Han, Yali, Guo, Jingjing, Hao, Zhen, Zhu, Xi, Zhou, Qiong, Du, Xiadong, Lu, Gang, He, Shenyi, Luo, Yingquan
Format: Article
Language:English
Subjects:
Animal Systematics/Taxonomy/Biogeography
Animals
Antigens
Antigens, Protozoan - chemistry
Antigens, Protozoan - genetics
Antigens, Protozoan - immunology
Biomedical and Life Sciences
Biomedicine
Cloning, Molecular
Computational Biology - methods
Computer programs
constructed plasmids
Construction
Dynamic structural analysis
Ecology
Epitopes
Epitopes, B-Lymphocyte - genetics
Epitopes, B-Lymphocyte - immunology
Epitopes, T-Lymphocyte - genetics
Epitopes, T-Lymphocyte - immunology
expression
Gene Expression
HEK293 Cells
Homeotherms
Humans
Immune Sera
Internet
Lymphocytes
Lymphocytes B
Lymphocytes T
Medical Microbiology
Mice
Microbiology
Molecular chains
Molecular Conformation
Molecular dynamics
Molecular structure
Parasitology
Plasmids - genetics
Polymerase chain reaction
Protein structure
Proteins
Protozoa
Protozoan Proteins - chemistry
Protozoan Proteins - genetics
Protozoan Proteins - immunology
ROP20
Secondary structure
secondary structures
Software
Structural analysis
Three dimensional models
Toxoplasma - chemistry
Toxoplasma - immunology
Toxoplasma gondii
Toxoplasmosis
Western blotting
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Description
Summary:Toxoplasma gondii infects almost all the warm-blooded animals. ROP20 protein is expressed in the rhoptry of Toxoplasma gondii . In this study, the secondary structure of ROP20 was analyzed using SMART software. We constructed and analyzed the 3D model of ROP20 protein using SWISS-MODEL online procedure and Visual Molecular Dynamics (VMD) software. The structure analysis fully indicated that ROP20 protein is an important member of the ROP family. Furthermore, We used DNASTAR software and Epitope Database online service to analyze liner-B cell epitopes and T-cell epitopes of ROP20 protein. All the analysis results of ROP20 protein can provide positive information on treatment and vaccine for toxoplasmosis. Moreover, ROP20 gene was obtained from PCR, and a recombinant eukaryotic expression vector (pEGFP-C1-ROP20) was constructed in the following study. After restriction enzyme digestion, the constructed plasmid was transfected into HEK 293-T cells. The RT-PCR result indicated that the recombinant plasmid could transcribe successfully in HEK 293-T cell. The results of western blotting indicated the expressed proteins can be recognized by anti-STAg mouse sera.
ISSN:1230-2821
1896-1851
DOI:10.1515/ap-2018-0028