Forensic SNP Genotyping with SNaPshot: Development of a Novel In‐house SBE Multiplex SNP Assay

This study introduces a newly developed in‐house SNaPshot single‐base extension (SBE) multiplex assay for forensic single nucleotide polymorphism (SNP) genotyping of fresh and degraded samples. The assay was validated with fresh blood samples from four different populations. In addition, altogether...

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Bibliographic Details
Published in:Journal of forensic sciences 2018-11, Vol.63 (6), p.1824-1829
Main Authors: Zar, Mian Sahib, Shahid, Ahmad Ali, Shahzad, Muhammad Saqib, Shin, Kyoung‐Jin, Lee, Hwan Young, Lee, Sang‐Seob, Israr, Muhammad, Wiegand, Peter, Kulstein, Galina
Format: Article
Language:English
Subjects:
Assaying
Bone and Bones - chemistry
Crime
DNA Degradation, Necrotic
DNA Fingerprinting
forensic genetics
forensic genotyping
forensic science
Gene Frequency
Genotype
Genotyping Techniques - instrumentation
Human remains
Humans
multiplex development
Multiplexing
Polymerase Chain Reaction
Polymorphism
Polymorphism, Single Nucleotide
population genetics
Populations
single nucleotide polymorphism
single‐base extension
skeletal remains
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Summary:This study introduces a newly developed in‐house SNaPshot single‐base extension (SBE) multiplex assay for forensic single nucleotide polymorphism (SNP) genotyping of fresh and degraded samples. The assay was validated with fresh blood samples from four different populations. In addition, altogether 24 samples from skeletal remains were analyzed with the multiplex. Full SNP profiles could be obtained from 14 specimens, while ten remains showed partial SNP profiles. Minor allele frequencies (MAF) of bone samples and different populations were compared and used for association of skeletal remains with a certain population. The results reveal that the SNPs of the bone samples are genetically close to the Pathan population. The findings show that the new multiplex system can be utilized for SNP genotyping of degraded and forensic relevant skeletal material, enabling to provide additional investigative leads in criminal cases.
ISSN:0022-1198
1556-4029
DOI:10.1111/1556-4029.13783