Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells

Purpose The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the...

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Bibliographic Details
Published in:Pharmaceutical research 2018-06, Vol.35 (6), p.112-14, Article 112
Main Authors: Zayed, Gamal M., Kamal, Islam, Abdelhafez, Wael A., M. Alsharif, Fahd, Amin, Mohamed A., Shaykoon, Montaser Sh. A., Sarhan, Hatem A., Abdelsalam, Ahmed M.
Format: Article
Language:English
Subjects:
Acetic acid
Adenocarcinoma
Adsorption
Alkanes
Antibiotics, Antineoplastic - administration & dosage
Antibiotics, Antineoplastic - chemistry
Antibiotics, Antineoplastic - pharmacokinetics
Biochemistry
Biomedical and Life Sciences
Biomedical Engineering and Bioengineering
Biomedicine
Breast cancer
Breast Neoplasms - drug therapy
Breast Neoplasms - pathology
Cancer
Chemotherapy
Cytotoxicity
Doxorubicin
Doxorubicin - administration & dosage
Doxorubicin - chemistry
Doxorubicin - pharmacokinetics
Drug Carriers - chemistry
Drug Liberation
Drug Screening Assays, Antitumor
Drug therapy
Female
Functional groups
Gold - chemistry
Humans
Hydrogen-Ion Concentration
MCF-7 Cells
Medical Law
Metal Nanoparticles - chemistry
Nanoparticles
pH effects
Pharmacology/Toxicology
Pharmacy
Polyethylene glycol
Polyethylene Glycols - chemistry
Polyols
Research Paper
Static Electricity
Tumors
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Summary:Purpose The selective delivery of chemotherapeutic agent to the affected area is mainly dependent on the mode of drug loading within the delivery system. This study aims to compare the physical method to the chemical method on the efficiency of loading DOX.HCl to GNPs and the specific release of the loaded drug at certain tissue. Method Bifunctional polyethylene glycol with two different functionalities, the alkanethiol and the carboxyl group terminals, was synthesized. Then, DOX·HCl was covalently linked via hydrazone bond, a pH sensitive bond, to the carboxyl functional group and the produced polymer was used to prepare drug functionalized nanoparticles. Another group of GNPs was coated with carboxyl containing polymer; loading the drug into this system by the means of electrostatic adsorption. Finally, the prepared system were characterized with respect to size, shape and drug release in acetate buffer pH 5 and PBS pH 7.4 Also, the effect of DOX.HCl loaded systems on cell viability was assessed using MCF-7 breast cancer cell line. Results The prepared nanoparticles were spherical in shape, small in size and monodisperse. The release rate of the chemically bound drug in the acidic pH was higher than the electrostatically adsorbed one. Moreover, both systems show little release at pH 7.4. Finally, cytotoxicity profiles against human breast adenocarcinoma cell line (MCF-7) exhibited greater cytotoxicity of the chemically bound drug over the electrostatically adsorbed one. Conclusion Chemical binding of DOX·HCl to the carboxyl group of PEG coating GNPs selectively delivers high amount of drug to tumour-affected tissue which leads to reducing the unwanted effects of the drug in the non-affected ones.
ISSN:0724-8741
1573-904X
DOI:10.1007/s11095-018-2393-6