Endogenously generated amyloid β increases membrane fluidity in neural 2a cells

The effect of endogenously generated amyloid β on membrane fluidity was investigated in Neural 2a cells stably expressing Swedish mutant amyloid precursor protein （APPswe）. Membrane fluidity was studied by fluorescence polarizability using 1,6-Diphenyl-1,3,5-Hexatriene （DPH） as the fluorescence prob...

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Bibliographic Details
Published in:Chinese science bulletin 2009-02, Vol.54 (3), p.394-398
Main Authors: Niu, Ying, Sheng, BaiYang, Song, Bo, Liu, LingLing, Zhang, XiuFang, Zhao, NanMing, Gong, YanDao
Format: Article
Language:English
Subjects:
Articles/Biophysics
Chemistry/Food Science
Earth Sciences
Engineering
Humanities and Social Sciences
Life Sciences
multidisciplinary
Physics
Science
Science (multidisciplinary)
老年痴呆症
膜流动性
荧光极化
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Summary:The effect of endogenously generated amyloid β on membrane fluidity was investigated in Neural 2a cells stably expressing Swedish mutant amyloid precursor protein （APPswe）. Membrane fluidity was studied by fluorescence polarizability using 1,6-Diphenyl-1,3,5-Hexatriene （DPH） as the fluorescence probe. It was found that the membrane fluidity in APPswe cells was significantly higher than that in its wild type counterparts. Alleviating the effect of amyloid β either by y secretase activity inhibition or by amyloid antibody treatment decreased membrane fluidity, which indicated an important role of amyloid β in increasing membrane fluidity. Treatment using amyloid β channel blocker, tromethamine and NA4 suggested that channel formed by amyloid β on the cell membrane is a way through which amyloid β takes its membrane fluidizing effect.
ISSN:1001-6538
2095-9273
1861-9541
2095-9281
DOI:10.1007/s11434-008-0527-7