In situ biomonitoring of PAH-contaminated sediments using juvenile coho salmon ( Oncorhynchus kisutch)

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous marine and freshwater sediment contaminants. Extensive data exist to confirm that PAHs are toxic to aquatic receptors. However, limited information is available regarding the bioavailability and genotoxicity of sediment PAHs to aquatic organisms...

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Bibliographic Details
Published in:Ecotoxicology and environmental safety 2008-10, Vol.71 (2), p.454-464
Main Authors: Barbee, Gary C., Barich, John, Duncan, Bruce, Bickham, John W., Matson, Cole W., Hintze, Christopher J., Autenrieth, Robin L., Zhou, Guo-Dong, McDonald, Thomas J., Cizmas, Leslie, Norton, Dale, Donnelly, Kirby C.
Format: Article
Language:English
Subjects:
Animals
Biomarker
Biomarkers
Coho salmon
DNA Adducts
Ecotoxicity
Environmental Monitoring - methods
Fresh Water - chemistry
Freshwater
Geologic Sediments - chemistry
Liver - drug effects
Mutagenicity Tests
Oncorhynchus kisutch
Oncorhynchus kisutch - growth & development
PAH
Polycyclic Aromatic Hydrocarbons - analysis
Polycyclic Aromatic Hydrocarbons - toxicity
Sediment quality triad
Water Pollutants, Chemical - analysis
Water Pollutants, Chemical - toxicity
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Summary:Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous marine and freshwater sediment contaminants. Extensive data exist to confirm that PAHs are toxic to aquatic receptors. However, limited information is available regarding the bioavailability and genotoxicity of sediment PAHs to aquatic organisms. This study investigated an integrated biomonitoring approach using chemical analyses and biomarkers to characterize the bioavailability and genotoxicity of a complex PAH mixture in freshwater lake sediments associated with a former manufactured gas plant (MGP). Sediment PAH genotoxicity was assessed by flow cytometry (FCM), DNA adduct 32P-postlabeling, and erythrocyte micronuclei in juvenile coho salmon ( Oncorhynchus kisutch) caged in the water column. Significant PAH-induced genotoxicity was observed with FCM and 32P-postlabeling, but not with erythrocyte micronuclei. Chromosome damage in peripheral blood and hepatic DNA adducts correlated with sediment, but not water column PAH concentrations. Total hepatic DNA adducts in salmon caged nearest the former MGP facility was 39±6.5 (RAL×10 9), while salmon caged in a reference lake had 28±2.3 total hepatic DNA adducts per 10 9 nucleotides. These results indicate that in situ biomonitoring using biomarkers and caged fish can be a sensitive indicator of genotoxic PAHs in sediments.
ISSN:0147-6513
1090-2414
DOI:10.1016/j.ecoenv.2008.01.001