Effects of mancozeb, metalaxyl and tebuconazole on steroid production by bovine luteal cells in vitro

•Mancozeb, metalaxyl and tebuconazole can induce inhibition in luteal steroidogenesis.•Inhibition depends on both fungicide type applied and dose used in cell treatment.•Mancozeb has the most adverse effect on luteal cell steroidogenesis.•Steroid inhibition could be related to fungicide induced decr...

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Bibliographic Details
Published in:Environmental toxicology and pharmacology 2018-04, Vol.59, p.114-118
Main Authors: Atmaca, Nurgul, Arikan, Sevket, Essiz, Dinc, Kalender, Hakan, Simsek, Ozkan, Bilmen, Fatih Sultan, Kabakci, Ruhi
Format: Article
Language:English
Subjects:
Aeration
Agrochemicals
Cell culture
Corpus luteum
Culture media
Dependent variables
Fungi
Fungicide
Fungicides
Human exposure
Incubation
Livestock
Luteal cells
Mancozeb
Metalaxyl
Pesticides
Progesterone
Steroidogenesis
Synthesis
Tebuconazole
Toxicology
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Summary:•Mancozeb, metalaxyl and tebuconazole can induce inhibition in luteal steroidogenesis.•Inhibition depends on both fungicide type applied and dose used in cell treatment.•Mancozeb has the most adverse effect on luteal cell steroidogenesis.•Steroid inhibition could be related to fungicide induced decrease in enzyme activity. Mancozeb, metalaxyl and tebucanazole are widely used pesticides in agriculture and industry to treat plant pathogenic fungi. Livestock may be exposed to such substances by consuming contaminated plants. The present study was designed to evaluate the effects of these three fungicides on bovine luteal cell steroidogenesis. Luteal slices from mid-cycle corpus luteum were dissociated into single cell suspension in aerated (O2) culture media (DMEM/F12) by enzymatic digestion. The cells were incubated in newborn calf serum (10%) for 18 h and then with serum-free media containing mancozeb (0.01 μM, 0.1 μM, 1 μM), tebuconazole (1 μM, 10 μM, 100 μM) or metalaxyl (100 μM, 500 μM, 2500 μM) for additional 96 h. The medium was replaced on day 1 and 3; and the retrieved medium was stored at −20 °C until progesterone assay. Treatment of cells with three different fungicides induced dose dependent variable decrease in steroid synthesis during incubation periods. Incubation of cells with 1 μM mancozeb exhibited a 33% decline in steroid synthesis on day 3 and 48% decline on day 5 compared with controls. Treatment of cells with 100 μM tebuconazole and 500 μM metalaxyl resulted in a 65% and 31% decrease, respectively, in progesterone accumulation on day 5 of incubation. Fungicide induced suppressive effects on luteal steroidogenesis were as metalaxyl 
ISSN:1382-6689
1872-7077
DOI:10.1016/j.etap.2018.03.009