Relative Abundance of Proteins in Blood Plasma Samples from Patients with Chronic Cerebral Ischemia

A comparative protein profile analysis of 17 blood plasma samples from patients with ischemia and 20 samples from healthy volunteers was carried out using ultra-high resolution mass spectrometry. The analysis of measurements was performed using the proteomics search engine OMSSA. Normalized spectrum...

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Bibliographic Details
Published in:Journal of molecular neuroscience 2018-03, Vol.64 (3), p.440-448
Main Authors: Kaysheva, Anna L., Kopylov, Artur T., Ponomarenko, Elena A., Kiseleva, Olga I., Teryaeva, Nadezhda B., Potapov, Alexander A., Izotov, Alexander А., Morozov, Sergei G., Kudryavtseva, Valeria Yu, Archakov, Alexander I.
Format: Article
Language:English
Subjects:
Abundance
Biological properties
Biological samples
Biomedical and Life Sciences
Biomedicine
Blood coagulation
Blood plasma
Cell Biology
Clotting
Homeostasis
Immune response
Immune system
Innate immunity
Ischemia
Mass spectrometry
Mass spectroscopy
Neurochemistry
Neurology
Neurosciences
Patients
Plasma
Protein composition
Proteins
Proteomics
Relative abundance
Scientific imaging
Spectroscopy
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Summary:A comparative protein profile analysis of 17 blood plasma samples from patients with ischemia and 20 samples from healthy volunteers was carried out using ultra-high resolution mass spectrometry. The analysis of measurements was performed using the proteomics search engine OMSSA. Normalized spectrum abundance factor (NSAF) in the biological samples was assessed using SearchGUI. The findings of mass spectrometry analysis of the protein composition of blood plasma samples demonstrate that the depleted samples are quite similar in protein composition and relative abundance of proteins. By comparing them with the control samples, we have found a small group of 44 proteins characteristic of the blood plasma samples from patients with chronic cerebral ischemia. These proteins contribute to the processes of homeostasis maintenance, including innate immune response unfolding, the response of a body to stress, and contribution to the blood clotting cascade.
ISSN:0895-8696
1559-1166
DOI:10.1007/s12031-018-1040-3