Knockdown of fascin-1 expression suppresses cell migration and invasion of non-small cell lung cancer by regulating the MAPK pathway

Fascin-1 is a cytoskeletal protein and it can specifically bind to F-actin, it can be abnormally expressed in a variety of solid tumors. Fascin-1 was identified as a factor for poor prognosis in non-small cell lung cancer (NSCLC). However, the relevant molecular mechanisms are not yet fully understo...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2018-03, Vol.497 (2), p.694-699
Main Authors: Zhao, Da, Zhang, Tao, Hou, Xiao-Ming, Ling, Xiao-Ling
Format: Article
Language:English
Subjects:
Fascin-1
Invasion
MAPK signaling
Migration
NSCLC
Proliferation
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Summary:Fascin-1 is a cytoskeletal protein and it can specifically bind to F-actin, it can be abnormally expressed in a variety of solid tumors. Fascin-1 was identified as a factor for poor prognosis in non-small cell lung cancer (NSCLC). However, the relevant molecular mechanisms are not yet fully understood. In this study, the fascin-1 knockdown cells were produced by lentivirus infection, and then cell proliferation, invasion and cell migration assay were used to investigate the role of fascin-1 in NSCLC cells. The MAPK pathway related proteins were determined by western blot. In the current study, lentivirus-mediated fascin-1 knockdown significantly decreased the proliferation of NSCLC cells. Furthermore, fascin-1 silencing partly inhibited cell invasion and migration. Inhibition of fascin-1 decreased the activity of the MAPK pathway. Therefore, targeting fascin-1 may inhibit the growth and metastasis of NSCLC cells, which is a potentially effective therapeutic strategy for treating NSCLC. •The fascin-1 promotes NSCLC cells proliferation.•The fascin-1 promotes NSCLC cells invasion and migration.•The fascin-1 promotes the malignant phenotype by targeting MAPK signal pathway in NSCLC cells.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2018.02.134