Efficient visible light induced synthesis of silver nanoparticles by Penicillium polonicum ARA 10 isolated from Chetomorpha antennina and its antibacterial efficacy against Salmonella enterica serovar Typhimurium

The green synthesis of silver nanoparticles (AgNPs) using biological systems such as fungi has evolved to become an important area of nanobiotechnology. Herein, we report for the first time the light-induced extracellular synthesis of silver nanoparticles using algicolous endophytic fungus Penicilli...

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Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2018-03, Vol.180, p.175-185
Main Authors: Neethu, Sahadevan, Midhun, Sebastian Jose, Sunil, M.A., Soumya, Soman, Radhakrishnan, E.K., Jyothis, Mathew
Format: Article
Language:English
Subjects:
AgNP
Antiinfectives and antibacterials
Bacteria
Bactericidal efficacy
Biological evolution
Biosynthesis
Cell walls
Characterization
Endophytes
Filtrate
Fluorescence
Fourier transforms
Fungi
Infrared spectroscopy
Light effects
Lysis
Minimum inhibitory concentration
Molecular chains
Nanoparticles
Optimization
Penicillium
Penicillium polonicum
Proteins
Raman spectroscopy
Reaction time
Salmonella
Salmonella enterica
Scanning electron microscopy
Silver
Spectroscopy
Stability analysis
Synthesis
Transmission electron microscopy
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Summary:The green synthesis of silver nanoparticles (AgNPs) using biological systems such as fungi has evolved to become an important area of nanobiotechnology. Herein, we report for the first time the light-induced extracellular synthesis of silver nanoparticles using algicolous endophytic fungus Penicillium polonicum ARA 10, isolated from the marine green alga Chetomorpha antennina. Parametric optimization, including the concentration of AgNO3, fungal biomass, ratio of cell filtrate and AgNO3, pH, reaction time and presence of light, was done for rapid AgNPs production. The obtained silver nanoparticles (AgNPs) were characterized by UV–Visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy and Transmission electron microscopy (HRTEM-EDAX). The AgNPs showed a characteristic UV–visible peak at 430 nm with an average size of 10-15 nm. The NH stretches in FTIR indicate the presence of protein molecules. The Raman vibrational bands suggest that the molecules responsible for the reduction and stability of AgNPs were extracellular proteins produced by P.polonicum. Antibacterial evaluation of AgNPs against the major foodborne bacterial pathogen Salmonella enterica serovar Typhimurium MTCC 1251, was assessed by well diffusion, Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assay. Killing kinetic studies revealed complete killing of the bacterial cells within 4 h and the bactericidal nature of synthesized nanoparticles was confirmed by fluorescent microscopy and scanning electron microscopy. Furthermore, the bactericidal studies with Transmission electron microscopy (TEM) at different time intervals explored the presence of AgNPs in the cell wall of S.Typhimurium at about 30 min and the complete bacterial lysis was found at 24 h. The current research opens an insight into the green synthesis of AgNPs and the mechanism of bacterial lysis by direct damage to the cell wall. [Display omitted] •Efficacy of the marine endophytic fungus Penicillium polonicum ARA10 is proposed.•Antibacterial activity of biosynthesized AgNP was studied against Salmonella enterica Typhimurium.•Mechanism of bactericidal effects of AgNPs demonstrated.•Ultra-structural studies of nanoparticles-bacterial interactions were explored.
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2018.02.005