A point‐of‐care diagnostic system to influenza viruses using chip‐based ultra‐fast PCR

In order to diagnose the infectious disease from clinical samples, the various protocols such as culturing microorganism, rapid diagnostic test using chromatographic method, ELISA, conventional PCR are developed. Since a novel strain of avian influenza can be cross‐infected human as well as birds an...

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Bibliographic Details
Published in:Journal of medical virology 2018-06, Vol.90 (6), p.1019-1026
Main Authors: Kwon, Soon‐Hwan, Lee, Sujin, Jang, Jeyoun, Seo, Yujin, Lim, Hee‐Young
Format: Article
Language:English
Subjects:
Avian flu
Biological warfare
Birds
chip‐based PCR
Diagnostic software
Diagnostic systems
Enzyme-linked immunosorbent assay
EvaGreen
GeneChecker
Health risks
Humans
Infectious diseases
Influenza
Influenza A
influenza subtyping
Influenza, Human - diagnosis
Livestock
Molecular Diagnostic Techniques - methods
Orthomyxoviridae - genetics
Orthomyxoviridae - isolation & purification
POCT
Point-of-Care Systems
Polymerase Chain Reaction - methods
Reverse transcription
Test procedures
Time Factors
Virology
Viruses
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Summary:In order to diagnose the infectious disease from clinical samples, the various protocols such as culturing microorganism, rapid diagnostic test using chromatographic method, ELISA, conventional PCR are developed. Since a novel strain of avian influenza can be cross‐infected human as well as birds and livestock due to genetic reassortment, some strains of influenza such as H7N9 and H5N1 have emerged as a severe virus which can be threaten the health of poultry as well as human. Therefore, we explored the development of simultaneously and rapid diagnostic tool for seasonal influenza (A/H1N1, A/H3N2, B) and highly pathogenic avian influenza (A/H5N1, A/H7N9). We analyzed the unique nucleotide sequences of influenza types including three seasonal influenza, A/H7N9, and A/H5N1, and distinguished each type of influenza and diagnosed through One Step RT‐PCR. In the results, Chip‐based PCR technique can be diagnosed rapidly and directly from naked eye with EvaGreen the influenza also respiratory specimens within 23 min 15 s, including reverse transcription. The Chip‐based PCR is a point‐of‐care system, and it is expected to reduce diagnosis time and to develop a diagnostic kit. Furthermore the Chip based PCR technique can be used for high risk pathogen in bioterror and/or biological warfare in the field.
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.25046