Physicochemical characterization of pectinase activity from Bacillus spp. and their accessory role in synergism with crude xylanase and commercial cellulase in enzyme cocktail mediated saccharification of agrowaste biomass

Aim The aim of this study was to evaluate the physicochemical properties of the crude pectinase activity from three Bacillus isolates of ruminant dung origin and study their synergism with crude xylanases from the same Bacillus spp. and a commercial cellulase to evaluate their accessory role in impr...

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Bibliographic Details
Published in:Journal of applied microbiology 2018-05, Vol.124 (5), p.1147-1163
Main Authors: Thite, V.S., Nerurkar, A.S.
Format: Article
Language:English
Subjects:
Agricultural wastes
agrowaste biomass
Bacillus
Bacillus altitudinis
Bacillus safensis
Biomass
Cell culture
Cellulase
Dung
Enzymatic activity
Enzyme activity
enzyme cocktail
Enzymes
Filtrate
Hydrolase
Pectate lyase
Pectin
Pectin lyase
Pectinase
pectinase activity
pH effects
Physicochemical properties
Plant biomass
Saccharification
Substrate specificity
Substrates
sugarcane bagasse
Synergism
Temperature effects
Viscosity
Xylanase
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Summary:Aim The aim of this study was to evaluate the physicochemical properties of the crude pectinase activity from three Bacillus isolates of ruminant dung origin and study their synergism with crude xylanases from the same Bacillus spp. and a commercial cellulase to evaluate their accessory role in improved biomass saccharification. Methods and Results Pectinolytic crude culture filtrate obtained from three ruminant dung isolates, Bacillus safensis M35, Bacillus altitudinis R31 and Bacillus altitudinis J208, on crude pectin containing medium possessed polygalacturonate hydrolase, pectate lyase and pectin lyase activities. Studies regarding their stability under various temperature and pH conditions revealed their mild acidic to alkaline and mesophilic nature with enzyme activity falling within the pH range 6·0–9·0 and temperature range 30–60°C. The pectinase activity was categorized as endolytic as it brought about ~50% reduction in relative viscosity of pectic polymer within initial 10 min of incubation. Synergism of pectinase activity with crude xylanase activities and/or commercial cellulase was clearly demonstrated as ~1·6 to ~1·9‐fold increase in agrowaste biomass saccharification was obtained confirming the role of pectinases as accessory enzymes. Conclusion Synergism of the broad‐spectrum endopectinase activity obtained from three Bacillus isolates with accessory crude xylanases from the same isolates and commercial cellulase enhanced the agrowaste saccharification and confirmed the accessory role of crude pectinase as they formed an efficient enzyme cocktail functioning in a contributive manner for improvement of agrowaste biomass saccharification. Significance and Impact of the study Mesophilic crude endopectinases obtained from Bacillus spp. isolated from ruminant dung possessed activity in broad pH and temperature ranges as well as broad substrate specificity. Moreover, their synergism with crude xylanase and Primfast®200 cellulase demonstrated the potential to form efficient enzyme cocktail for application in plant biomass saccharification process.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.13718