Production of isoamyl acetate with immobilized Staphylococcus simulans lipase in a solvent-free system

A non-commercial immobilized lipase preparation from Staphylococcus simulans was used to catalyze the esterification reaction between acetic acid and isoamyl alcohol to synthetize isoamyl acetate (banana flavour) in pure substrate conditions (without addition of any organic solvent). The effects of...

Full description

Saved in:
Bibliographic Details
Published in:Enzyme and microbial technology 2006-04, Vol.38 (6), p.788-794
Main Authors: Ghamgui, Hanen, Karra-Chaâbouni, Maha, Bezzine, Sofiane, Miled, Nabil, Gargouri, Youssef
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Esterification
Fundamental and applied biological sciences. Psychology
Immobilized lipase
Isoamyl acetate
Musa
Solvent-free system
Staphylococcus simulans
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A non-commercial immobilized lipase preparation from Staphylococcus simulans was used to catalyze the esterification reaction between acetic acid and isoamyl alcohol to synthetize isoamyl acetate (banana flavour) in pure substrate conditions (without addition of any organic solvent). The effects of various reaction parameters such as the amount of lipase, the molar ratio of acetic acid as compared to isoamyl alcohol and the amount of initially added water were investigated. The optimum conditions for achieving 64% conversion during 8 h of incubation are 60 IU of immobilized lipase, an acid/alcohol of 2 molar ratio and an initial addition of water of 10% (w/w). No significant loss of the immobilized lipase activity was observed after four cycles of use of the immobilized enzymatic preparation.
ISSN:0141-0229
1879-0909
DOI:10.1016/j.enzmictec.2005.08.011