CRISPR-based assay for the molecular identification of highly prevalent Salmonella serotypes

•Innovative serotyping method for highly prevalent Salmonella serotypes.•Use of CRISPR spacers suitable for serotype determination.•CRISPR spacer content diversity associated with geographical origin of the strains.•Validation study combining experimental and in silico investigations.

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Bibliographic Details
Published in:Food microbiology 2018-05, Vol.71, p.8-16
Main Authors: Bugarel, Marie, Bakker, Henk den, Grout, Joel, Vignaud, Marie-Leone, Loneragan, Guy H., Fach, Patrick, Brisabois, Anne
Format: Article
Language:English
Subjects:
Bacteriological Techniques - methods
Biomarkers
Clustered Regularly Interspaced Short Palindromic Repeats
DNA, Bacterial - analysis
Genes, Bacterial - genetics
Molecular Diagnostic Techniques - methods
Multilocus Sequence Typing - methods
Polymerase Chain Reaction
Prevalence
Salmonella - classification
Salmonella - genetics
Salmonella - isolation & purification
Salmonella - pathogenicity
Salmonella Infections - diagnosis
Salmonella Infections - microbiology
Serogroup
Serotyping - methods
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Description
Summary:•Innovative serotyping method for highly prevalent Salmonella serotypes.•Use of CRISPR spacers suitable for serotype determination.•CRISPR spacer content diversity associated with geographical origin of the strains.•Validation study combining experimental and in silico investigations.
ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2017.03.016