Recent trends and analytical challenges in plant bioactive peptide separation, identification and validation

Interest in research into bioactive peptides (BPs) is growing because of their health-promoting ability. Several bioactivities have been ascribed to peptides, including antioxidant, antihypertensive and antimicrobial properties. As they can be produced from precursor proteins, the investigation of B...

Full description

Saved in:
Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2018-06, Vol.410 (15), p.3425-3444
Main Authors: Piovesana, Susy, Capriotti, Anna Laura, Cavaliere, Chiara, La Barbera, Giorgia, Montone, Carmela Maria, Zenezini Chiozzi, Riccardo, Laganà, Aldo
Format: Article
Language:English
Subjects:
Agricultural wastes
Analgesics, Opioid - analysis
Analgesics, Opioid - isolation & purification
Analgesics, Opioid - pharmacology
Analytical Chemistry
Animals
Antihypertensive Agents - analysis
Antihypertensive Agents - isolation & purification
Antihypertensive Agents - pharmacology
Antihypertensives
Antioxidants
Antioxidants - analysis
Antioxidants - isolation & purification
Antioxidants - pharmacology
Biochemistry
Biological activity
Botanical research
Byproducts
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Chromatography - methods
Dietary supplements
Discovery of Bioactive Compounds
Environmental impact
Food
Food production
Food Science
Graphical representations
Health promotion
Humans
Hydrolysis
Identification
Industrial wastes
Laboratory Medicine
Mass Spectrometry - methods
Mathematical analysis
Meat
Milk
Monitoring/Environmental Analysis
Peptides
Peptides - analysis
Peptides - isolation & purification
Peptides - pharmacology
Plant proteins
Plant Proteins - chemistry
Plants - chemistry
Protein research
Proteins
Review
Separation
Ultrafiltration - methods
Vegetables
Vegetables - chemistry
Workflow
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Interest in research into bioactive peptides (BPs) is growing because of their health-promoting ability. Several bioactivities have been ascribed to peptides, including antioxidant, antihypertensive and antimicrobial properties. As they can be produced from precursor proteins, the investigation of BPs in foods is becoming increasingly popular. For the same reason, production of BPs from by-products has also emerged as a possible means of reducing waste and recovering value-added compounds suitable for functional food production and supplements. Milk, meat and fish are the most investigated sources of BPs, but vegetable-derived peptides are also of interest. Vegetables are commonly consumed, and agro-industrial wastes constitute a cheap, large and lower environmental impact source of proteins. The use of advanced analytical techniques for separation and identification of peptides would greatly benefit the discovery of new BPs. In this context, this review provides an overview of the most recent applications in BP investigations for vegetable food and by-products. The most important issues regarding peptide isolation and separation, by single or multiple chromatographic techniques, are discussed. Additionally, problems connected with peptide identification in plants and non-model plants are discussed regarding the particular case of BP identification. Finally, the issue of peptide validation to confirm sequence and bioactivity is presented. Graphical representation of the analytical workflow needed for investigation of bioactive peptides and applied to vegetables and vegetable wastes Graphical Abstract
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-018-0852-x