Identification of the Minimal Glycotope of Streptococcus pneumoniae 7F Capsular Polysaccharide using Synthetic Oligosaccharides

Streptococcus pneumoniae causes life‐threatening diseases including meningitis, pneumonia and sepsis. Existing glycoconjugate vaccines based on purified capsular polysaccharides are widely used and help to prevent millions of deaths every year. Herein, the total syntheses of oligosaccharides resembl...

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Bibliographic Details
Published in:Chemistry : a European journal 2018-03, Vol.24 (16), p.4181-4187
Main Authors: Ménová, Petra, Sella, Mauro, Sellrie, Katrin, Pereira, Claney L., Seeberger, Peter H.
Format: Article
Language:English
Subjects:
Capsular polysaccharides
Chemistry
Epitopes
Epitopes - immunology
Glycan
glycan arrays
Glycoconjugates - immunology
Humans
Meningitis
minimal glycotopes
Oligosaccharides
Pneumococcal Vaccines - chemistry
Pneumococcal Vaccines - immunology
Pneumococcal Vaccines - therapeutic use
Pneumonia
Polysaccharides
Polysaccharides - chemistry
Polysaccharides - immunology
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - immunology
Saccharides
Sepsis
Serogroup
serotype 7F
Streptococcus infections
Streptococcus pneumoniae
Streptococcus pneumoniae - chemistry
total synthesis
Vaccines
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Summary:Streptococcus pneumoniae causes life‐threatening diseases including meningitis, pneumonia and sepsis. Existing glycoconjugate vaccines based on purified capsular polysaccharides are widely used and help to prevent millions of deaths every year. Herein, the total syntheses of oligosaccharides resembling portions of the S. pneumoniae serotype 7F (ST7F) capsular polysaccharide repeating unit are reported. To define minimal glycan epitopes, glycan microarrays containing the synthetic oligosaccharides were used to screen human reference serum and revealed that both side chains of the ST7F play a key role in antigen recognition. The identification of protective minimal epitopes is vital to design efficient semi‐ and fully‐synthetic glycoconjugate vaccines. Fighting pneumonia: Synthetic oligosaccharides resembling the Streptococcus pneumoniae serotype 7F capsular polysaccharide repeating unit were printed onto glycan microarrays. Screening of human reference serum helped to define minimal epitopes where both side chains on the central glycan are essential. Identification of protective minimal epitopes is key to the design of semi‐ and fully‐synthetic glycoconjugate vaccines.
ISSN:0947-6539
1521-3765
DOI:10.1002/chem.201705379