Engineering of protein assemblies within cells

•Engineering of microcompartment proteins under intracellular overexpression conditions.•In vivo-encapsulation of exogenous materials within a protein-cage.•Approaches to produce 3D crystals of proteins in living cells.•Design of protein interactions for self-assembling materials. Recent achievement...

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Bibliographic Details
Published in:Current opinion in structural biology 2018-08, Vol.51, p.1-8
Main Authors: Nguyen, Tien K, Ueno, Takafumi
Format: Article
Language:English
Subjects:
Biotechnology
Gene Expression
Intracellular Space
Models, Molecular
Protein Binding
Protein Conformation
Protein Engineering
Proteins - chemistry
Proteins - genetics
Proteins - metabolism
Recombinant Proteins
Structure-Activity Relationship
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Summary:•Engineering of microcompartment proteins under intracellular overexpression conditions.•In vivo-encapsulation of exogenous materials within a protein-cage.•Approaches to produce 3D crystals of proteins in living cells.•Design of protein interactions for self-assembling materials. Recent achievements in development of protein assembles within cells have extended biosupramolecular composites into a new era with versatile applications in the fields of biomaterial and biotechnology. Using methods with biological and physicochemical routes has made this era of research more interesting and challenging. Further advances in protein engineering have facilitated efficient fabrication of supramolecular complexes within living cells. Here, we provide a review of recent efforts to engineer protein assemblies within cells and describe the promising properties of these assemblies.
ISSN:0959-440X
1879-033X
DOI:10.1016/j.sbi.2017.12.005