Backscattered electron imaging and electron backscattered diffraction in the study of bacterial attachment to titanium alloy structure

Summary The application of secondary electron (SE) imaging, backscattered electron imaging (BSE) and electron backscattered diffraction (EBSD) was investigated in this work to study the bacterial adhesion and proliferation on a commercially pure titanium (cp Ti) and a Ti6Al4V alloy (Ti 64) with resp...

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Bibliographic Details
Published in:Journal of microscopy (Oxford) 2018-04, Vol.270 (1), p.53-63
Main Authors: WANG, ANQI, JONES, IAN P., LANDINI, GABRIEL, MEI, JUNFA, TSE, YAU Y., LI, YUE X., KE, LINNAN, HUANG, YUANLI, LIU, LI, WANG, CHUNREN, SAMMONS, RACHEL L.
Format: Article
Language:English
Subjects:
Adhesion
Alloys
Aluminum
Bacteria
Bacteria adhesion
Bacterial Adhesion
Boundaries
Chemical composition
Diffraction
E coli
Electron backscatter diffraction
Electron imaging
Electron microscopy
Environmental Microbiology
Grain boundaries
grain boundary
Grain orientation
Gram-Negative Bacteria - growth & development
Gram-Positive Bacteria - growth & development
Heavy metals
Microorganisms
Microscopy, Electron
Microstructure
Scanning electron microscopy
Solid phases
Staphylococcus epidermidis
Statistical methods
Statistics
Strains (organisms)
Streptococcus sanguinis
Substrates
Surface Properties
Titanium
Titanium alloys
Titanium base alloys
Vanadium
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Summary:Summary The application of secondary electron (SE) imaging, backscattered electron imaging (BSE) and electron backscattered diffraction (EBSD) was investigated in this work to study the bacterial adhesion and proliferation on a commercially pure titanium (cp Ti) and a Ti6Al4V alloy (Ti 64) with respect to substrate microstructure and chemical composition. Adherence of Gram‐positive Staphylococcus epidermidis 11047 and Streptococcus sanguinis GW2, and Gram‐negative Serratia sp. NCIMB 40259 and Escherichia coli 10418 was compared on cp Ti, Ti 64, pure aluminium (Al) and vanadium (V). The substrate microstructure and the bacterial distribution on these metals were characterised using SE, BSE and EBSD imaging. It was observed that titanium alloy‐phase structure, grain boundaries and grain orientation did not influence bacterial adherence or proliferation at microscale. Adherence of all four strains was similar on cp Ti and Ti 64 surfaces whilst inhibited on pure Al. This work establishes a nondestructive and straight‐forward statistical method to analyse the relationship between microbial distribution and metal alloy structure. Lay description The application of scanning electron microscopy and its various modes was investigated in this work to study the bacterial adhesion and proliferation on polished pure titanium and its alloy (Ti6Al4V) to investigate the influence of substrate microstructure and chemical composition. Adherence of two Gram‐positive bacterial strains, and two Gram‐negative ones was compared and the bacterial distribution with respects to substrate microstructure quantified. It was observed that titanium alloy‐phase structure, grain boundaries and grain orientation did not influence bacterial adherence or proliferation at microscale. Adherence of all four strains was similar on the two titanium surfaces whilst inhibited on pure aluminium. This work establishes a nondestructive and straight‐forward statistical method to analyse the relationship between microbial distribution and metal alloy structure.
ISSN:0022-2720
1365-2818
DOI:10.1111/jmi.12649